Overview
- External ontology
- mediadive.medium:203 (source link unavailable) — METHANOTHERMUS MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_203_modified_for_dsm_3537', 'source': 'KOMODO', 'source_id': 'komodo.medium:203_3537', 'original_category': 'bacterial'}
- {'name': 'medium_203_modified_for_dsm_3538', 'source': 'KOMODO', 'source_id': 'komodo.medium:203_3538', 'original_category': 'bacterial'}
- pH
- 6.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium203.pdf
Composition
flowchart LR
CultureMech_001302["methanothermus_medium"]
ing0_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001302 --> ing0_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing0_NiCl2_x_6_H2O -.-> CHEBI_34887
ing1_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001302 --> ing1_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing1_FeSO4_x_7_H2O -.-> CHEBI_75836
ing2_Na2SO4["Na2SO4"]
CultureMech_001302 --> ing2_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing2_Na2SO4 -.-> CHEBI_32149
ing3_Sodium_resazurin["Sodium resazurin"]
CultureMech_001302 --> ing3_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Sodium_resazurin -.-> CHEBI_8806
ing4_Na2CO3["Na2CO3"]
CultureMech_001302 --> ing4_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing4_Na2CO3 -.-> CHEBI_29377
ing5_Yeast_extract["Yeast extract"]
CultureMech_001302 --> ing5_Yeast_extract
ing6_Trypticase_peptone["Trypticase peptone"]
CultureMech_001302 --> ing6_Trypticase_peptone
ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001302 --> ing7_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_x_9_H2O -.-> CHEBI_76209
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001302 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_K2HPO4["K2HPO4"]
CultureMech_001302 --> ing9_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing9_K2HPO4 -.-> CHEBI_131527
ing10_KH2PO4["KH2PO4"]
CultureMech_001302 --> ing10_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing10_KH2PO4 -.-> CHEBI_63036
ing11__NH4_2SO4["(NH4)2SO4"]
CultureMech_001302 --> ing11__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing11__NH4_2SO4 -.-> CHEBI_62946
ing12_NaCl["NaCl"]
CultureMech_001302 --> ing12_NaCl
CHEBI_26710["CHEBI:26710"]
ing12_NaCl -.-> CHEBI_26710
ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001302 --> ing13_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001302 --> ing14_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001302 --> ing15_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
ing16_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001302 --> ing16_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing16_MnSO4_x_H2O -.-> CHEBI_86364
ing17_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001302 --> ing17_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing17_CoSO4_x_7_H2O -.-> CHEBI_91244
ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001302 --> ing18_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001302 --> ing19_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
ing20_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001302 --> ing20_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing20_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing21_H3BO3["H3BO3"]
CultureMech_001302 --> ing21_H3BO3
CHEBI_33118["CHEBI:33118"]
ing21_H3BO3 -.-> CHEBI_33118
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001302 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001302 --> ing23_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001302 --> ing24_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing25_Biotin["Biotin"]
CultureMech_001302 --> ing25_Biotin
CHEBI_15956["CHEBI:15956"]
ing25_Biotin -.-> CHEBI_15956
ing26_Folic_acid["Folic acid"]
CultureMech_001302 --> ing26_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing26_Folic_acid -.-> CHEBI_27470
ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001302 --> ing27_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing28_Thiamine_HCl["Thiamine HCl"]
CultureMech_001302 --> ing28_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing28_Thiamine_HCl -.-> CHEBI_49105
ing29_Riboflavin["Riboflavin"]
CultureMech_001302 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_001302 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.030991079999999997 G_PER_L |
CHEBI:34887
nickel dichloride
|
[Merged 2 duplicates: 0.00099108, 0.03] |
| 2 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.10198216 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.00198216, 0.1] |
| 3 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.36967 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 4 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.00049554 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 5 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.99108 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 6 | Yeast extract | 1.98216 G_PER_L | ||
| 7 | Trypticase peptone | 1.98216 G_PER_L | ||
| 8 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.49554 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.49554 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 11 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 12 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
6 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 13 |
NaCl
Cl.Na 58.443 g/mol
|
13.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 12.0, 1.0] |
| 14 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
5.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 2.4, 3.0] |
| 15 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.7000000000000002 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 1.6, 0.1] |
| 16 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 17 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 18 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 19 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 20 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 21 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 22 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 24 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 25 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 26 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 27 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 28 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 29 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 31 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 32 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 33 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 34 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 35 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients except carbonate, yeast extract, Trypticase peptone, vitamins, sulfide and cysteine, then adjust pH to 6.0 with sulfuric acid. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into serum bottles to 30% of their volume and autoclave. Thereafter, add by injection yeast extract, Trypticase peptone, vitamins (sterilized by filtration), sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 6.5.
- After inoculation pressurize bottles to 2 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- methanothermus_medium (CultureMech:001302)
- medium_203_modified_for_dsm_3537 (CultureMech:004299)
- medium_203_modified_for_dsm_3538 (CultureMech:004300)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.492641Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 203 |
| 2026-02-06T05:10:32.331023Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:09.243965Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:19:03.033900Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001302 |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.914182+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOTHERMUS MEDIUM" to "methanothermus_medium" for consistent matching |
| 2026-06-05T07:41:32.906680+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.478837+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:31.617094+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: methanothermus_medium, medium_203_modified_for_dsm_3537, medium_203_modified_for_dsm_3538 |
| 2026-08-06T03:04:31.617167+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 41bda6a4141e1c09f981e79d344e74284cc124d6b0ceda5ee163683b1b28e821 |