Overview
- External ontology
- mediadive.medium:236 (source link unavailable) — B12 - MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium236.pdf
Composition
flowchart LR
CultureMech_001338["b12_medium"]
ing0_Casein_hydrolysate["Casein hydrolysate"]
CultureMech_001338 --> ing0_Casein_hydrolysate
ing1_K2HPO4["K2HPO4"]
CultureMech_001338 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001338 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001338 --> ing3_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
ing4_Glycerol["Glycerol"]
CultureMech_001338 --> ing4_Glycerol
CHEBI_17754["CHEBI:17754"]
ing4_Glycerol -.-> CHEBI_17754
ing5_Asparagine["Asparagine"]
CultureMech_001338 --> ing5_Asparagine
CHEBI_22653["CHEBI:22653"]
ing5_Asparagine -.-> CHEBI_22653
ing6_Vitamin_B12["Vitamin B12"]
CultureMech_001338 --> ing6_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing6_Vitamin_B12 -.-> CHEBI_176843
ing7_Agar["Agar"]
CultureMech_001338 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein hydrolysate | 6 G_PER_L | ||
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 5 |
Glycerol
C3H8O3 92.094 g/mol
|
2 G_PER_L |
CHEBI:17754
glycerol
|
|
| 6 |
Asparagine
C4H8N2O3 132.119 g/mol
|
0.15 G_PER_L |
CHEBI:22653
asparagine
|
|
| 7 | Vitamin B12 | 4e-05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- b12_medium (CultureMech:001338)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.573791Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 236 |
| 2026-03-13T06:19:03.281902Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001338 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.188203+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "B12 - MEDIUM" to "b12_medium" for consistent matching |
| 2026-06-05T07:40:50.178337+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.682590+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:24.522434+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 83618cce5da2a18c231e649ce0cf27fc0048e8175dc829a75cd2a4e97778c345 |