Overview
- External ontology
- mediadive.medium:271 (source link unavailable) — ACIDITHIOBACILLUS (APH) MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_271_modified_for_dsm_11477', 'source': 'KOMODO', 'source_id': 'komodo.medium:271_11477', 'original_category': 'bacterial'}
- {'name': 'medium_271_modified_for_dsm_11478', 'source': 'KOMODO', 'source_id': 'komodo.medium:271_11478', 'original_category': 'bacterial'}
- {'name': 'medium_271_modified_for_dsm_24413', 'source': 'KOMODO', 'source_id': 'komodo.medium:271_24413', 'original_category': 'bacterial'}
- pH
- 2.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium271.pdf
Composition
flowchart LR
CultureMech_001370["acidithiobacillus_aph_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_001370 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_K2HPO4["K2HPO4"]
CultureMech_001370 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001370 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_KCl["KCl"]
CultureMech_001370 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_001370 --> ing4_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing4_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001370 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.02 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
8 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Preparation
- Dissolve ingredients and adjust pH to 2.0 with 10 N H2SO4. Sterilize the medium by filtration. Alternatively, autoclave separately the basal medium (pH adjusted to 2.0) and the ferrous sulfate (8.00 g FeSO4 x 7 H2O in 50 ml 0.1 N H2SO4, pH not adjusted, in a sealed vessel under nitrogen gas atmosphere).
- Note: Incubate statically without shaking.
Target organisms
No target organisms are recorded.
Provenance
Merged from 4 source records.
- acidithiobacillus_aph_medium (CultureMech:001370)
- medium_271_modified_for_dsm_11477 (CultureMech:004696)
- medium_271_modified_for_dsm_11478 (CultureMech:004697)
- medium_271_modified_for_dsm_24413 (CultureMech:004698)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.624612Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 271 |
| 2026-03-13T06:19:03.442474Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001370 |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.371537+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACIDITHIOBACILLUS (APH) MEDIUM" to "acidithiobacillus_aph_medium" for consistent matching |
| 2026-06-05T07:40:47.324087+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.606022+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:21.935405+00:00 | recipe-merger | Merged 4 duplicate recipes into canonical record | Sources: acidithiobacillus_aph_medium, medium_271_modified_for_dsm_11477, medium_271_modified_for_dsm_11478, medium_271_modified_for_dsm_24413 |
| 2026-08-06T03:05:21.935455+00:00 | merge_recipes.py | MERGED_RECIPES | merged 4 source recipe(s) on fingerprint b0450bbf4774e9eace3d4fcbfb1677b2c61a08ebc5f5e3eca8d354a8a8ba09f3 |