Overview
- External ontology
- mediadive.medium:276 (source link unavailable) — HALOMONAS MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_276_modified_for_dsm_11845', 'source': 'KOMODO', 'source_id': 'komodo.medium:276_11845', 'original_category': 'bacterial'}
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium276.pdf
Composition
flowchart LR
CultureMech_001373["halomonas_medium"]
ing0_NaCl["NaCl"]
CultureMech_001373 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_Casamino_acids["Casamino acids"]
CultureMech_001373 --> ing1_Casamino_acids
ing2_Proteose_peptone_no__3["Proteose peptone no. 3"]
CultureMech_001373 --> ing2_Proteose_peptone_no__3
ing3_Yeast_extract["Yeast extract"]
CultureMech_001373 --> ing3_Yeast_extract
ing4_Na3_citrate["Na3-citrate"]
CultureMech_001373 --> ing4_Na3_citrate
CHEBI_53258["CHEBI:53258"]
ing4_Na3_citrate -.-> CHEBI_53258
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001373 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_K2HPO4["K2HPO4"]
CultureMech_001373 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_001373 --> ing7_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing7_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing8_Agar["Agar"]
CultureMech_001373 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
80 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 | Casamino acids | 7.5 G_PER_L | ||
| 3 | Proteose peptone no. 3 | 5 G_PER_L | ||
| 4 | Yeast extract | 1 G_PER_L | ||
| 5 |
Na3-citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.05 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(if necessary) |
Preparation
- Before autoclaving adjust pH to 7.5 with NaOH
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- halomonas_medium (CultureMech:001373)
- medium_276_modified_for_dsm_11845 (CultureMech:004704)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.633589Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 276 |
| 2026-01-28T19:48:35.370352Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:03.471338Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001373 |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.403715+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOMONAS MEDIUM" to "halomonas_medium" for consistent matching |
| 2026-06-05T07:41:07.880882+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.739724+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:59.553897+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:37.228107+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: halomonas_medium, medium_276_modified_for_dsm_11845 |
| 2026-08-06T03:05:37.228149+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 4ae5a2303cd4b4245fd4fd0bfc9cff637281003fd465943a14565b40739be58b |