methanocaldococcus_villosus_medium

CultureMech:001379 archaea DEFINED LIQUID
Original name: METHANOCALDOCOCCUS VILLOSUS MEDIUM

Overview

External ontology
mediadive.medium:282a (source link unavailable) — METHANOCALDOCOCCUS VILLOSUS MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'methanocaldococcus_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:282', 'original_category': 'archaea'}
  • {'name': 'methanococcus_jannaschii_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:282', 'original_category': 'archaea'}
  • {'name': 'for_dsm_22549', 'source': 'KOMODO', 'source_id': 'komodo.medium:282.1', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_22612', 'source': 'KOMODO', 'source_id': 'komodo.medium:282.2', 'original_category': 'bacterial'}
  • {'name': 'medium_282_modified_for_dsm_16983', 'source': 'KOMODO', 'source_id': 'komodo.medium:282_16983', 'original_category': 'bacterial'}
  • {'name': 'medium_282_modified_for_dsm_5666', 'source': 'KOMODO', 'source_id': 'komodo.medium:282_5666', 'original_category': 'bacterial'}
pH
6.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium282a.pdf

Composition

flowchart LR
    CultureMech_001379["methanocaldococcus_villosus_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001379 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001379 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001379 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001379 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001379 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_001379 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001379 --> ing6_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing6_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing7_NaCl["NaCl"]
    CultureMech_001379 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_001379 --> ing8_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing8_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing9_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001379 --> ing9_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Sodium_resazurin -.-> CHEBI_8806
    ing10_NaHCO3["NaHCO3"]
    CultureMech_001379 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001379 --> ing11_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001379 --> ing12_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_x_9_H2O -.-> CHEBI_76209
    ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001379 --> ing13_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing14_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001379 --> ing14_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing14_MnSO4_x_H2O -.-> CHEBI_86364
    ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001379 --> ing15_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001379 --> ing16_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001379 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001379 --> ing18_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing19_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001379 --> ing19_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing19_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing20_H3BO3["H3BO3"]
    CultureMech_001379 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001379 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001379 --> ing22_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing22_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing23_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001379 --> ing23_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing23_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing24_Biotin["Biotin"]
    CultureMech_001379 --> ing24_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing24_Biotin -.-> CHEBI_15956
    ing25_Folic_acid["Folic acid"]
    CultureMech_001379 --> ing25_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing25_Folic_acid -.-> CHEBI_27470
    ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001379 --> ing26_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing27_Thiamine_HCl["Thiamine HCl"]
    CultureMech_001379 --> ing27_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing27_Thiamine_HCl -.-> CHEBI_49105
    ing28_Riboflavin["Riboflavin"]
    CultureMech_001379 --> ing28_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing28_Riboflavin -.-> CHEBI_17015
    ing29_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001379 --> ing29_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing29_Nicotinic_acid -.-> CHEBI_15940
    CultureMech_001379 --> more["...4 more"]

Ingredients (34)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.13834 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.23834 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.13834, 0.1]
3 NH4Cl
Cl.H4N 53.492 g/mol
0.247036 G_PER_L CHEBI:31206
ammonium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.35968 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 3.35968, 3.0]
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.05138 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 KCl
Cl.K 74.551 g/mol
0.326087 G_PER_L CHEBI:32588
potassium chloride
7 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.030494070999999998 G_PER_L CHEBI:34887
nickel dichloride
 [Merged 2 duplicates: 0.000494071, 0.03]
8 NaCl
Cl.Na 58.443 g/mol
30.6443 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 29.6443, 1.0]
9 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.00988142 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
10 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000494071 G_PER_L CHEBI:8806
Resazurin
11 NaHCO3
CHO3.Na 84.006 g/mol
0.988142 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.494071 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
13 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.494071 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
14 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
15 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
16 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
17 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
18 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
19 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
20 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
21 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
23 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
24 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
25 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
26 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
27 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
28 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
29 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
30 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
31 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
32 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
33 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
34 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium into anoxic tubes to 30% of their volume under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 6.0, if necessary.
  2. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure.
  3. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 7 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:10.647041Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 282a
2026-01-28T19:48:11.380279Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:17.490340Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:08:46.351574Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:03.534407Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001379
2026-03-17T06:29:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.472109+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOCALDOCOCCUS VILLOSUS MEDIUM" to "methanocaldococcus_villosus_medium" for consistent matching
2026-06-05T07:41:31.104461+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.812991+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:30.040456+00:00 recipe-merger Merged 7 duplicate recipes into canonical record Sources: methanocaldococcus_medium, methanocaldococcus_villosus_medium, methanococcus_jannaschii_medium, for_dsm_22549, for_dsm_22612, medium_282_modified_for_dsm_16983, medium_282_modified_for_dsm_5666
2026-08-06T03:04:30.040559+00:00 merge_recipes.py MERGED_RECIPES merged 7 source recipe(s) on fingerprint ad19012458a74570d1edf3ec6d563c55a8b5afec1f20840978c4622c471c19cb