Overview
- External ontology
- mediadive.medium:319 (source link unavailable) — SPOROHALOBACTER LORTETII MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium319.pdf
Composition
flowchart LR
CultureMech_001420["sporohalobacter_lortetii_medium"]
ing0_Casamino_acids["Casamino acids"]
CultureMech_001420 --> ing0_Casamino_acids
ing1_Nutrient_broth["Nutrient broth"]
CultureMech_001420 --> ing1_Nutrient_broth
ing2_Yeast_extract["Yeast extract"]
CultureMech_001420 --> ing2_Yeast_extract
ing3_L_Glutamic_acid["L-Glutamic acid"]
CultureMech_001420 --> ing3_L_Glutamic_acid
CHEBI_16015["CHEBI:16015"]
ing3_L_Glutamic_acid -.-> CHEBI_16015
ing4_NaCl["NaCl"]
CultureMech_001420 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_KCl["KCl"]
CultureMech_001420 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001420 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_001420 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001420 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001420 --> ing9_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001420 --> ing10_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001420 --> ing11_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001420 --> ing12_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
ing13_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001420 --> ing13_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing13_MnSO4_x_H2O -.-> CHEBI_86364
ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001420 --> ing14_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001420 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001420 --> ing16_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
ing17_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001420 --> ing17_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing17_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing18_H3BO3["H3BO3"]
CultureMech_001420 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001420 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001420 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001420 --> ing21_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001420 --> ing22_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing23_Biotin["Biotin"]
CultureMech_001420 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_Folic_acid["Folic acid"]
CultureMech_001420 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001420 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Thiamine_HCl["Thiamine HCl"]
CultureMech_001420 --> ing26_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing26_Thiamine_HCl -.-> CHEBI_49105
ing27_Riboflavin["Riboflavin"]
CultureMech_001420 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_001420 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001420 --> ing29_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing29_Calcium_D_____pantothenate -.-> CHEBI_31345
CultureMech_001420 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casamino acids | 1.97433 G_PER_L | ||
| 2 | Nutrient broth | 1.97433 G_PER_L | ||
| 3 | Yeast extract | 1.97433 G_PER_L | ||
| 4 |
L-Glutamic acid
C5H9NO4 147.13 g/mol
|
3.94867 G_PER_L |
CHEBI:16015
L-glutamic acid
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
104.653 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 103.653, 1.0] |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.740375 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.10197433 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.00197433, 0.1] |
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000493583 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.493583 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
9.87167 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 11 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.75252 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 3.65252, 0.1] |
| 12 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 13 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 14 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 15 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 17 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 18 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 22 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 23 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 27 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 30 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 31 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 32 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 33 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except cysteine, magnesium chloride, calcium chloride and vitamins), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Thereafter, add and dissolve cysteine, adjust pH to 6.5 and dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add magnesium chloride and calcium chloride from sterile anoxic stock solutions autoclaved under 100% N2 gas and vitamins from a filter-sterilized stock solution prepared under N2 gas.
- Note: For agar medium add 20.00 g/l agar, 5.00 g/l CaCO3, and 2.00 g/l soluble starch.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- sporohalobacter_lortetii_medium (CultureMech:001420)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.778223Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 319 |
| 2026-01-28T19:48:25.852516Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-06T05:10:34.753310Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:12.927016Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:03.980771Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001420 |
| 2026-03-17T06:30:20.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.962225+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SPOROHALOBACTER LORTETII MEDIUM" to "sporohalobacter_lortetii_medium" for consistent matching |
| 2026-06-05T07:41:46.633513+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:50.533886+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:47:11.692411+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:56.369291+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 23895480b269db3e5587695d56baf36790a59f59e81c6f4741f3ceefe14ad5bb |