oxobacter_pfennigii_medium

CultureMech:001436 bacterial COMPLEX LIQUID
Original name: OXOBACTER PFENNIGII MEDIUM

Overview

External ontology
mediadive.medium:336 (source link unavailable) — OXOBACTER PFENNIGII MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'clostridium_pfennigii_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:336', 'original_category': 'bacterial'}
pH range
{'min': 7.0, 'max': 7.2}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium336.pdf

Composition

flowchart LR
    CultureMech_001436["oxobacter_pfennigii_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001436 --> ing0_Yeast_extract
    ing1_Na_vanillate["Na-vanillate"]
    CultureMech_001436 --> ing1_Na_vanillate
    CHEBI_132748["CHEBI:132748"]
    ing1_Na_vanillate -.-> CHEBI_132748
    ing2_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001436 --> ing2_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing2_Sodium_resazurin -.-> CHEBI_8806
    ing3_Na2CO3["Na2CO3"]
    CultureMech_001436 --> ing3_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing3_Na2CO3 -.-> CHEBI_29377
    ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001436 --> ing4_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001436 --> ing5_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing5_Na2S_x_9_H2O -.-> CHEBI_76209
    ing6_KH2PO4["KH2PO4"]
    CultureMech_001436 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001436 --> ing7_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing8_NaCl["NaCl"]
    CultureMech_001436 --> ing8_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing8_NaCl -.-> CHEBI_26710
    ing9_NH4Cl["NH4Cl"]
    CultureMech_001436 --> ing9_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing9_NH4Cl -.-> CHEBI_31206
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001436 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_HCl["HCl"]
    CultureMech_001436 --> ing11_HCl
    CHEBI_17883["CHEBI:17883"]
    ing11_HCl -.-> CHEBI_17883
    ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001436 --> ing12_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing13_ZnCl2["ZnCl2"]
    CultureMech_001436 --> ing13_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing13_ZnCl2 -.-> CHEBI_49976
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001436 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_H3BO3["H3BO3"]
    CultureMech_001436 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001436 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001436 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001436 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001436 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_Vitamin_B12["Vitamin B12"]
    CultureMech_001436 --> ing20_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing20_Vitamin_B12 -.-> CHEBI_176843
    ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001436 --> ing21_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing22_D_____biotin["D-(+)-biotin"]
    CultureMech_001436 --> ing22_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing22_D_____biotin -.-> CHEBI_15956
    ing23_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001436 --> ing23_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing23_Nicotinic_acid -.-> CHEBI_15940
    ing24_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001436 --> ing24_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing24_Calcium_pantothenate -.-> CHEBI_31345
    ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001436 --> ing25_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing26_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001436 --> ing26_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing26_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 Yeast extract 2.05761 G_PER_L
2 Na-vanillate
C8H7O4.Na 190.13 g/mol
2.05761 G_PER_L CHEBI:132748
sodium vanillate
3 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
4 Na2CO3
CO3.2Na 105.988 g/mol
50 G_PER_L CHEBI:29377
sodium carbonate
5 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
30 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
6 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
30 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
7 KH2PO4
H2O4P.K 136.084 g/mol
10 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
8 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
6.6 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
9 NaCl
Cl.Na 58.443 g/mol
8 G_PER_L CHEBI:26710
sodium chloride
10 NH4Cl
Cl.H4N 53.492 g/mol
8 G_PER_L CHEBI:31206
ammonium chloride
11 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
12 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
13 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
14 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
21 Vitamin B12 0.1 G_PER_L CHEBI:176843
vitamin B12
22 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.08 G_PER_L CHEBI:30753
4-aminobenzoic acid
23 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
24 Nicotinic acid
C6H5NO2 123.111 g/mol
0.2 G_PER_L CHEBI:15940
nicotinic acid
25 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate
26 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.3 G_PER_L CHEBI:30961
pyridoxine hydrochloride
27 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.2 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate

Preparation

  1. Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, distribute under same gas atmosphere 8.9 ml medium per Hungate-type tube and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas atmosphere and sterilized by filtration. Autoclave solutions D and E under 100% N2 gas. To complete the medium add appropriate amounts of the solutions B to E to the sterile solution A. Adjust pH of complete medium to 7.0 - 7.2, if necessary.
  2. Adjust pH to 6.9 before autoclaving.
  3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
  4. Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:10.841021Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 336
2026-03-13T06:19:04.181203Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001436
2026-03-17T06:30:33.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.176589+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "OXOBACTER PFENNIGII MEDIUM" to "oxobacter_pfennigii_medium" for consistent matching
2026-05-20T06:02:01.720406+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2}
2026-06-05T07:41:40.714888+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.836041+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:28.470150+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: clostridium_pfennigii_medium, oxobacter_pfennigii_medium
2026-08-06T03:05:28.470195+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint c4f9647d476a7ffe7366937fe493f242fe02987ef78cc56808fc60dbb68ac30e