acidithiobacillus_medium

Overview

External ontology

mediadive.medium:35 (source link unavailable) — ACIDITHIOBACILLUS MEDIUM

Applications

• Microbial cultivation

pH

4.2

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium35.pdf

Composition

flowchart LR
    CultureMech_001459["acidithiobacillus_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_001459 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001459 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001459 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001459 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Sulfur["Sulfur"]
    CultureMech_001459 --> ing4_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing4_Sulfur -.-> CHEBI_26833

Ingredients (5)

Preparation

1. Dissolve all ingredients, except the sulfur and adjust the pH to 4.2, then autoclave. For sterilization place the sulfur in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium.
2. Note: Incubate statically without shaking.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• acidithiobacillus_medium (CultureMech:001459)