pyrococcus_medium

Overview

External ontology

mediadive.medium:377 (source link unavailable) — PYROCOCCUS MEDIUM

Applications

• Microbial cultivation

pH

6.5

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium377.pdf

Composition

flowchart LR
    CultureMech_001478["pyrococcus_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001478 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001478 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001478 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_KCl["KCl"]
    CultureMech_001478 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NaBr["NaBr"]
    CultureMech_001478 --> ing4_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing4_NaBr -.-> CHEBI_63004
    ing5_H3BO3["H3BO3"]
    CultureMech_001478 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_001478 --> ing6_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing6_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing7__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001478 --> ing7__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing7__NH4_2SO4 -.-> CHEBI_62946
    ing8_Citric_acid["Citric acid"]
    CultureMech_001478 --> ing8_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing8_Citric_acid -.-> CHEBI_30769
    ing9_KI["KI"]
    CultureMech_001478 --> ing9_KI
    CHEBI_8346["CHEBI:8346"]
    ing9_KI -.-> CHEBI_8346
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001478 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_KH2PO4["KH2PO4"]
    CultureMech_001478 --> ing11_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing11_KH2PO4 -.-> CHEBI_63036
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001478 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001478 --> ing13_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing13_Sodium_resazurin -.-> CHEBI_8806
    ing14_Sulfur["Sulfur"]
    CultureMech_001478 --> ing14_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing14_Sulfur -.-> CHEBI_26833
    ing15_Peptone["Peptone"]
    CultureMech_001478 --> ing15_Peptone
    ing16_Yeast_extract["Yeast extract"]
    CultureMech_001478 --> ing16_Yeast_extract
    ing17_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001478 --> ing17_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing17_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing18_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001478 --> ing18_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing18_MnSO4_x_H2O -.-> CHEBI_86364
    ing19_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001478 --> ing19_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing19_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing20_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001478 --> ing20_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing20_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001478 --> ing21_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001478 --> ing22_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing23_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001478 --> ing23_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing23_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001478 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001478 --> ing25_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001478 --> ing26_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing27_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001478 --> ing27_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing27_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (28)

Preparation

1. Dissolve ingredients except for sulfur, peptone, yeast extract, and sulfide, adjust the pH to 6.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials that already contain the appropriate amount of sulfur. Sterilize the medium by autoclaving at <b>110°C</b> for 20 min. After sterilization, add peptone and yeast extract from sterile stock solutions prepared under 100% N2 gas atmosphere and sulfide from a neutralized stock solution.
2. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
3. The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121°C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle.

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

• Preferred term

  Pyrococcus furiosus

  Term

  Id

  NCBITaxon:2261

  Label

  Pyrococcus furiosus

  Strain modifications

  • Modification type

    ADAPTATION

    Target

    cellulose utilization

    Description

    serially adapted for growth on CF11 cellulose

  Growth metrics

  • Doubling time minutes

    64.0

    Evidence

    • Reference

      PMID:21421788

      Supports

      SUPPORT

      Snippet

      P. furiosus was serially adapted for growth on CF11 cellulose and H₂ production, which is the first reported instance of hyperthermophilic growth on cellulose, with a doubling time of 64 min.

      Explanation

      Auto-applied from literature query: backfill PMID:21421788

    Is max attainment

    False

    Growth mode

    BATCH

    Nutrient overrides

    • Role

      CARBON_SOURCE

      Source

      CF11 cellulose

      Is sole source

      True

Recipe variants

• Name

  cf11_cellulose_adapted_pyrococcus_furiosus

  Description

  Serially adapted Pyrococcus furiosus growth on CF11 cellulose.

  Modifications

  • Use Pyrococcus furiosus serially adapted for cellulose utilization.
  • Use CF11 cellulose as the growth substrate.

  Purpose

  Capture cellulose-adapted hyperthermophilic growth evidence under the parent Pyrococcus Medium record.

  Evidence

  • Reference

    PMID:21421788

    Supports

    SUPPORT

    Snippet

    P. furiosus was serially adapted for growth on CF11 cellulose and H₂ production, which is the first reported instance of hyperthermophilic growth on cellulose, with a doubling time of 64 min.

    Explanation

    Literature evidence supports a CF11-cellulose-adapted growth variant for Pyrococcus furiosus under the parent Pyrococcus Medium record.

Provenance

Merged from 1 source record.

• pyrococcus_medium (CultureMech:001478)