pyrococcus_st04_medium

CultureMech:001480 archaea COMPLEX LIQUID
Original name: PYROCOCCUS ST04 MEDIUM

Overview

External ontology
mediadive.medium:377b (source link unavailable) — PYROCOCCUS ST04 MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'pyrococcus_staphylothermus_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:377', 'original_category': 'archaea'}
  • {'name': 'for_dsm_19918', 'source': 'KOMODO', 'source_id': 'komodo.medium:377.1', 'original_category': 'bacterial'}
  • {'name': 'medium_377_modified_for_dsm_12428', 'source': 'KOMODO', 'source_id': 'komodo.medium:377_12428', 'original_category': 'bacterial'}
  • {'name': 'medium_377_modified_for_dsm_13482', 'source': 'KOMODO', 'source_id': 'komodo.medium:377_13482', 'original_category': 'bacterial'}
  • {'name': 'medium_377_modified_for_dsm_15127', 'source': 'KOMODO', 'source_id': 'komodo.medium:377_15127', 'original_category': 'bacterial'}
pH
6.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium377b.pdf

Composition

flowchart LR
    CultureMech_001480["pyrococcus_st04_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001480 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001480 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001480 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_KCl["KCl"]
    CultureMech_001480 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NaBr["NaBr"]
    CultureMech_001480 --> ing4_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing4_NaBr -.-> CHEBI_63004
    ing5_H3BO3["H3BO3"]
    CultureMech_001480 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_001480 --> ing6_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing6_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing7__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001480 --> ing7__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing7__NH4_2SO4 -.-> CHEBI_62946
    ing8_Citric_acid["Citric acid"]
    CultureMech_001480 --> ing8_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing8_Citric_acid -.-> CHEBI_30769
    ing9_KI["KI"]
    CultureMech_001480 --> ing9_KI
    CHEBI_8346["CHEBI:8346"]
    ing9_KI -.-> CHEBI_8346
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001480 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_KH2PO4["KH2PO4"]
    CultureMech_001480 --> ing11_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing11_KH2PO4 -.-> CHEBI_63036
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001480 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001480 --> ing13_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing13_Sodium_resazurin -.-> CHEBI_8806
    ing14_Sulfur["Sulfur"]
    CultureMech_001480 --> ing14_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing14_Sulfur -.-> CHEBI_26833
    ing15_Peptone["Peptone"]
    CultureMech_001480 --> ing15_Peptone
    ing16_Yeast_extract["Yeast extract"]
    CultureMech_001480 --> ing16_Yeast_extract
    ing17_NaHCO3["NaHCO3"]
    CultureMech_001480 --> ing17_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing17_NaHCO3 -.-> CHEBI_32139
    ing18_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001480 --> ing18_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing18_Na2S_x_9_H2O -.-> CHEBI_76209
    ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001480 --> ing19_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing20_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001480 --> ing20_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing20_MnSO4_x_H2O -.-> CHEBI_86364
    ing21_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001480 --> ing21_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing21_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing22_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001480 --> ing22_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing22_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing23_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001480 --> ing23_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing23_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing24_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001480 --> ing24_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing24_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing25_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001480 --> ing25_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing25_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing26_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001480 --> ing26_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing26_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing27_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001480 --> ing27_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing27_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing28_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001480 --> ing28_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing28_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
14.5122 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 13.5122, 1.0]
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.41463 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 3.41463, 3.0]
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.68293 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 KCl
Cl.K 74.551 g/mol
0.321951 G_PER_L CHEBI:32588
potassium chloride
5 NaBr
Br.Na 102.894 g/mol
0.0487805 G_PER_L CHEBI:63004
sodium bromide
6 H3BO3
H3BO3 61.833 g/mol
0.0246341 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.0146341, 0.01]
7 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.00682927 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
8 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.0097561 G_PER_L CHEBI:62946
ammonium sulfate
9 Citric acid
C6H8O7 192.123 g/mol
0.00487805 G_PER_L CHEBI:30769
citric acid
10 KI
I.K 166.002 g/mol
4.87805e-05 G_PER_L CHEBI:8346
potassium iodide
11 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.831707 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.731707, 0.1]
12 KH2PO4
H2O4P.K 136.084 g/mol
0.487805 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03195122 G_PER_L CHEBI:34887
nickel dichloride
 [Merged 2 duplicates: 0.00195122, 0.03]
14 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000487805 G_PER_L CHEBI:8806
Resazurin
15 Sulfur
S 32.067 g/mol
29.2683 G_PER_L CHEBI:26833
sulfur atom
16 Peptone 4.87805 G_PER_L
17 Yeast extract 0.97561 G_PER_L
18 NaHCO3
CHO3.Na 84.006 g/mol
1.95122 G_PER_L CHEBI:32139
sodium hydrogencarbonate
19 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.487805 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
20 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
21 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
22 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
23 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
24 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
25 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
26 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
27 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
28 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
29 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Dissolve ingredients except sulfur, peptone, yeast extract, bicarbonate, and sulfide, adjust the pH to 6.8, and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Distribute medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials that already contain the appropriate amount of sulfur. Sterilize the medium by autoclaving at <b>110°C</b> for 20 min. After sterilization add peptone, yeast extract, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust the pH of the complete medium to 6.8, if necessary.
  2. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 6 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:10.955271Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 377b
2026-03-13T03:09:43.711501Z data-quality-cleanup-v1.0 Data quality fixes Merged 5 duplicate ingredient(s)
2026-03-13T06:19:04.516938Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001480
2026-03-17T06:30:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.545762+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PYROCOCCUS ST04 MEDIUM" to "pyrococcus_st04_medium" for consistent matching
2026-06-05T07:41:43.311832+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.670283+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:32.497021+00:00 recipe-merger Merged 6 duplicate recipes into canonical record Sources: pyrococcus_st04_medium, pyrococcus_staphylothermus_medium, for_dsm_19918, medium_377_modified_for_dsm_12428, medium_377_modified_for_dsm_13482, medium_377_modified_for_dsm_15127
2026-08-06T03:04:32.497114+00:00 merge_recipes.py MERGED_RECIPES merged 6 source recipe(s) on fingerprint 024a5468897fdc6effd682e859820caac130efb9a69d0add32b32ec3f9acb032