Overview
- External ontology
- mediadive.medium:379 (source link unavailable) — CHROMATIUM TEPIDUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium379.pdf
Composition
flowchart LR
CultureMech_001482["chromatium_tepidum_medium"]
ing0_Na2_EDTA["Na2-EDTA"]
CultureMech_001482 --> ing0_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing0_Na2_EDTA -.-> CHEBI_64734
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001482 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001482 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_001482 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_NaCl["NaCl"]
CultureMech_001482 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Ammonium_acetate["Ammonium acetate"]
CultureMech_001482 --> ing5_Ammonium_acetate
CHEBI_62947["CHEBI:62947"]
ing5_Ammonium_acetate -.-> CHEBI_62947
ing6_KH2PO4["KH2PO4"]
CultureMech_001482 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_NaHCO3["NaHCO3"]
CultureMech_001482 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001482 --> ing8_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_x_9_H2O -.-> CHEBI_76209
ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001482 --> ing9_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
ing10_ZnCl2["ZnCl2"]
CultureMech_001482 --> ing10_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing10_ZnCl2 -.-> CHEBI_49976
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001482 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_H3BO3["H3BO3"]
CultureMech_001482 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001482 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001482 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001482 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001482 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
CultureMech_001482 --> ing17_VOSO4_x_2_H2O
CHEBI_87009["CHEBI:87009"]
ing17_VOSO4_x_2_H2O -.-> CHEBI_87009
ing18_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001482 --> ing18_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing18_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
5.21 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
[Merged 2 duplicates: 0.01, 5.2] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.4 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
0.4 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
Ammonium acetate
C2H3O2.H4N 77.083 g/mol
|
0.0005 G_PER_L |
CHEBI:62947
ammonium acetate
|
|
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.0005 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
1 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 10 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 11 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.188 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
VOSO4 x 2 H2O
2H2O.O4S.OV 199.036 g/mol
|
0.03 G_PER_L |
CHEBI:87009
vanadyl sulfate dihydrate
|
|
| 19 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.002 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Prepare bicarbonate and sulfide as separate solutions. Sterilize all solutions by autoclaving. After cooling mix all three solutions, adding the sulfide last. Adjust pH to 7.0 and dispense in completely filled screw-capped tubes or bottles. Incubate at 500 - 1000 Lux light intensity.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- chromatium_tepidum_medium (CultureMech:001482)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.961999Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 379 |
| 2026-03-13T03:08:57.509258Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:04.534587Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001482 |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.565774+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CHROMATIUM TEPIDUM MEDIUM" to "chromatium_tepidum_medium" for consistent matching |
| 2026-06-05T07:40:53.589403+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.576499+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:27.705377+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ca0f90af14562a03bfd1d8b9dd7fd0b8ce25d7991f0692d77ce2552e6d9dc7c2 |