Overview
- External ontology
- mediadive.medium:380 (source link unavailable) — MAGNETOSPIRILLUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.7
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium380.pdf
Composition
flowchart LR
CultureMech_001484["magnetospirillum_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001484 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NaNO3["NaNO3"]
CultureMech_001484 --> ing1_NaNO3
CHEBI_63005["CHEBI:63005"]
ing1_NaNO3 -.-> CHEBI_63005
ing2_L____Tartaric_acid["L(+)-Tartaric acid"]
CultureMech_001484 --> ing2_L____Tartaric_acid
CHEBI_15671["CHEBI:15671"]
ing2_L____Tartaric_acid -.-> CHEBI_15671
ing3_Succinic_acid["Succinic acid"]
CultureMech_001484 --> ing3_Succinic_acid
CHEBI_15741["CHEBI:15741"]
ing3_Succinic_acid -.-> CHEBI_15741
ing4_Na_acetate["Na-acetate"]
CultureMech_001484 --> ing4_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing4_Na_acetate -.-> CHEBI_32954
ing5_Yeast_extract["Yeast extract"]
CultureMech_001484 --> ing5_Yeast_extract
ing6_Agar["Agar"]
CultureMech_001484 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_001484 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_Na_thioglycolate["Na-thioglycolate"]
CultureMech_001484 --> ing8_Na_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing8_Na_thioglycolate -.-> CHEBI_86481
ing9_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001484 --> ing9_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing9_Nitrilotriacetic_acid -.-> CHEBI_44557
ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001484 --> ing10_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
ing11_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001484 --> ing11_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing11_MnSO4_x_H2O -.-> CHEBI_86364
ing12_NaCl["NaCl"]
CultureMech_001484 --> ing12_NaCl
CHEBI_26710["CHEBI:26710"]
ing12_NaCl -.-> CHEBI_26710
ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001484 --> ing13_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001484 --> ing14_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001484 --> ing15_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001484 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001484 --> ing17_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
ing18_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001484 --> ing18_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing18_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing19_H3BO3["H3BO3"]
CultureMech_001484 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001484 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001484 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001484 --> ing22_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing22_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing23_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001484 --> ing23_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing23_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing24_Vitamin_B12["Vitamin B12"]
CultureMech_001484 --> ing24_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing24_Vitamin_B12 -.-> CHEBI_176843
ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001484 --> ing25_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
ing26_D_____biotin["D-(+)-biotin"]
CultureMech_001484 --> ing26_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing26_D_____biotin -.-> CHEBI_15956
ing27_Nicotinic_acid["Nicotinic acid"]
CultureMech_001484 --> ing27_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing27_Nicotinic_acid -.-> CHEBI_15940
ing28_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_001484 --> ing28_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing28_Calcium_pantothenate -.-> CHEBI_31345
ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001484 --> ing29_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
CultureMech_001484 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.674603 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NaNO3
NO3.Na 84.994 g/mol
|
0.119048 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 3 |
L(+)-Tartaric acid
C4H6O6 150.086 g/mol
|
0.367063 G_PER_L |
CHEBI:15671
L-tartaric acid
|
|
| 4 |
Succinic acid
C4H6O4 118.088 g/mol
|
0.367063 G_PER_L |
CHEBI:15741
succinic acid
|
|
| 5 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.0496032 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 6 | Yeast extract | 0.0992064 G_PER_L | ||
| 7 | Agar | 1.28968 G_PER_L |
CHEBI:2509
agar
|
Optional ingredient (for semi-solid medium) |
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000496032 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
|
0.0496032 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
|
| 10 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 11 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 12 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 13 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 14 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 15 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 16 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 19 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 24 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 25 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 26 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 27 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 28 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 29 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 30 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.3 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 31 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.2 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 32 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
2.7 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 33 | (-)-Quinic acid | 1.9 G_PER_L |
Preparation
- Dissolve ingredients (except thioglycolate and vitamins) in the order given and adjust pH to 6.75 with NaOH.
- Preparation of liquid medium: Sparge medium with 100% N2 gas for 30 -45 min and dispense under the same gas atmosphere into anoxic Hungate-type tubes to 50% of their volume. Seal vials with screw caps and gas tight butyl rubber closures. Autoclave at 121°C for 15 min. Before inoculation add thioglycolate and vitamins from stock solutions prepared under 100% N2 gas and filter-sterilized. Then add sterile air (with hypodermic syringe through the rubber closure) to a concentration of ca. 1% (v/v) O2 in the vial (e.g., add 1 ml air to a Hungate-type tube of 16 ml total volume).
- Preparation of semi solid medium: Supplement medium with agar, bring medium to the boil and cool under 100% N2 gas atmosphere. Dispense under same gas atmosphere aliquots of 10 ml semi-solid medium into Hungate-type tubes. Prior to inoculation add thioglycolate from a 0.5% (w/v) stock solution, freshly prepared under 100% N2 gas and filter-sterilized. Then add sterile air (with hypodermic syringe through the rubber closure) to a concentration of ca. 1% (v/v) in the vial.
- Note: Prior to inoculation media should be slightly pink in color. Strongly reduced conditions will not support growth of microaerophilic Magnetospirillum species. Use as inoculum 10% (v/v). Incubate tubes with medium without agitation in an inclined position. During growth O2 will be consumed and the pH will increase. For cultivation of magnetic cells we recommend preparation of liquid medium, while semi-solid medium is more suitable for demonstration of microaerophilic band formation and storage.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
- Sterilize by filtration under 100% N2 gas atmosphere.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- magnetospirillum_medium (CultureMech:001484)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.964878Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 380 |
| 2026-03-13T06:19:04.552409Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001484 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.585243+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MAGNETOSPIRILLUM MEDIUM" to "magnetospirillum_medium" for consistent matching |
| 2026-06-05T07:41:12.540721+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.795512+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:41.642249+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint dc0f15960f931799dcde2afe7196c71d12afbecdcc2c6c258c499288d2a89021 |