methanococcus_voltae_medium (CultureMech:001498)

Overview

External ontology

mediadive.medium:391 (source link unavailable) — METHANOCOCCUS VOLTAE MEDIUM

Applications

Microbial cultivation

Synonyms

{'name': 'for_dsm_4254', 'source': 'KOMODO', 'source_id': 'komodo.medium:391.1', 'original_category': 'bacterial'}
{'name': 'for_strain_dsm_4310', 'source': 'KOMODO', 'source_id': 'komodo.medium:391.2', 'original_category': 'bacterial'}
{'name': 'wmc_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:391', 'original_category': 'bacterial'}

pH

7.2

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium391.pdf

Composition

flowchart LR
    CultureMech_001498["methanococcus_voltae_medium"]
    ing0_Na_acetate["Na-acetate"]
    CultureMech_001498 --> ing0_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing0_Na_acetate -.-> CHEBI_32954
    ing1_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001498 --> ing1_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing1_Sodium_resazurin -.-> CHEBI_8806
    ing2_Na2CO3["Na2CO3"]
    CultureMech_001498 --> ing2_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing2_Na2CO3 -.-> CHEBI_29377
    ing3_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001498 --> ing3_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing3_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001498 --> ing4_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing4_Na2S_x_9_H2O -.-> CHEBI_76209
    ing5_NaCl["NaCl"]
    CultureMech_001498 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001498 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001498 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_KCl["KCl"]
    CultureMech_001498 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_NH4Cl["NH4Cl"]
    CultureMech_001498 --> ing9_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing9_NH4Cl -.-> CHEBI_31206
    ing10_K2HPO4["K2HPO4"]
    CultureMech_001498 --> ing10_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing10_K2HPO4 -.-> CHEBI_131527
    ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001498 --> ing11_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing12_L_Leucine["L-Leucine"]
    CultureMech_001498 --> ing12_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing12_L_Leucine -.-> CHEBI_15603
    ing13_L_Isoleucine["L-Isoleucine"]
    CultureMech_001498 --> ing13_L_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing13_L_Isoleucine -.-> CHEBI_17191
    ing14_Pantothenate["Pantothenate"]
    CultureMech_001498 --> ing14_Pantothenate
    CHEBI_16454["CHEBI:16454"]
    ing14_Pantothenate -.-> CHEBI_16454
    ing15_Casamino_acids["Casamino acids"]
    CultureMech_001498 --> ing15_Casamino_acids
    ing16_Yeast_extract["Yeast extract"]
    CultureMech_001498 --> ing16_Yeast_extract
    ing17_L_Tryptophan["L-Tryptophan"]
    CultureMech_001498 --> ing17_L_Tryptophan
    CHEBI_16828["CHEBI:16828"]
    ing17_L_Tryptophan -.-> CHEBI_16828
    ing18_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001498 --> ing18_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing18_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing19_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001498 --> ing19_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing19_MnSO4_x_H2O -.-> CHEBI_86364
    ing20_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001498 --> ing20_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing20_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing21_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001498 --> ing21_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing21_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001498 --> ing22_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing23_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001498 --> ing23_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing23_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing24_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001498 --> ing24_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing24_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing25_H3BO3["H3BO3"]
    CultureMech_001498 --> ing25_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing25_H3BO3 -.-> CHEBI_33118
    ing26_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001498 --> ing26_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing26_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing27_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001498 --> ing27_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing27_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing28_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001498 --> ing28_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing28_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing29_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001498 --> ing29_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing29_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (30)

#	Ingredient	Concentration	Ontology	Notes
1	Na-acetate C2H3O2.Na 82.034 g/mol	1 G_PER_L	CHEBI:32954 sodium acetate
2	Sodium resazurin C12H7NO4 229.191 g/mol	0.0005 G_PER_L	CHEBI:8806 Resazurin
3	Na2CO3 CO3.2Na 105.988 g/mol	1.5 G_PER_L	CHEBI:29377 sodium carbonate
4	L-Cysteine HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.5 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate
5	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.5 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate
6	NaCl Cl.Na 58.443 g/mol	41.0 G_PER_L	CHEBI:26710 sodium chloride	[Merged 2 duplicates: 40.0, 1.0]
7	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	5.6 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate
8	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	3.7 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	[Merged 2 duplicates: 0.7, 3.0]
9	KCl Cl.K 74.551 g/mol	0.68 G_PER_L	CHEBI:32588 potassium chloride
10	NH4Cl Cl.H4N 53.492 g/mol	0.5 G_PER_L	CHEBI:31206 ammonium chloride
11	K2HPO4 HO4P.2K 174.174 g/mol	0.28 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
12	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.38 G_PER_L	CHEBI:86158 calcium chloride dihydrate	[Merged 2 duplicates: 0.28, 0.1]
13	L-Leucine C6H13NO2 131.175 g/mol	5 G_PER_L	CHEBI:15603 L-leucine
14	L-Isoleucine C6H13NO2 131.175 g/mol	10 G_PER_L	CHEBI:17191 L-isoleucine
15	Pantothenate C9H16NO5 218.229 g/mol	0.1 G_PER_L	CHEBI:16454 pantothenate
16	Casamino acids	100 G_PER_L
17	Yeast extract	50 G_PER_L
18	L-Tryptophan C11H12N2O2 204.229 g/mol	1 G_PER_L	CHEBI:16828 L-tryptophan
19	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	1.5 G_PER_L	CHEBI:44557 nitrilotriacetic acid
20	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.5 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate
21	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.1 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate
22	CoSO4 x 7 H2O Co.7H2O.O4S 281.101 g/mol	0.18 G_PER_L	CHEBI:91244 cobalt(2+) sulfate heptahydrate
23	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.18 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate
24	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.01 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate
25	AlK(SO4)2 x 12 H2O Al.12H2O.K.2O4S 474.386 g/mol	0.02 G_PER_L	CHEBI:86465 potassium aluminium sulfate dodecahydrate
26	H3BO3 H3BO3 61.833 g/mol	0.01 G_PER_L	CHEBI:33118 boric acid
27	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.01 G_PER_L	CHEBI:75213 sodium molybdate dihydrate
28	NiCl2 x 6 H2O Cl2Ni 129.599 g/mol	0.03 G_PER_L	CHEBI:34887 nickel dichloride
29	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	0.0003 G_PER_L	CHEBI:131361 disodium selenite pentahydrate
30	Na2WO4 x 2 H2O 2H2O.2Na.O4W 329.846 g/mol	0.0004 G_PER_L	CHEBI:63939 sodium tungstate dihydrate

Preparation

Dissolve ingredients except TYC solution, carbonate, cystein and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add TYC solution, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.2, if necessary.
May be stored frozen without sterilization.
Prepare and autoclave under 100% N2 gas.
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 4 source records.

methanococcus_voltae_medium (CultureMech:001498)
KOMODO_391-1_For_DSM_4254 (CultureMech:005173)
for_strain_dsm_4310 (CultureMech:005174)
wmc_medium (CultureMech:005175)

Timestamp	Curator	Action	Notes
2026-01-26T17:53:11.010456Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 391
2026-01-28T19:48:22.899665Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:07.846201Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 3 duplicate ingredient(s)
2026-03-13T06:19:04.701409Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001498
2026-03-17T06:30:14.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.754283+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "METHANOCOCCUS VOLTAE MEDIUM" to "methanococcus_voltae_medium" for consistent matching
2026-06-05T07:41:31.303443+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:47.674039+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24)	Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:47:06.890589+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:30.161157+00:00	recipe-merger	Merged 4 duplicate recipes into canonical record	Sources: methanococcus_voltae_medium, KOMODO_391-1_For_DSM_4254, for_strain_dsm_4310, wmc_medium
2026-08-06T03:04:30.161232+00:00	merge_recipes.py	MERGED_RECIPES	merged 4 source recipe(s) on fingerprint ce739f997feb13be849a1e54368a474fcafeeecc57e851a0b5caf3cb65107bd7