methanosphaera_cuniculi_medium

CultureMech:001514 archaea COMPLEX LIQUID
Original name: METHANOSPHAERA CUNICULI MEDIUM

Overview

External ontology
mediadive.medium:404 (source link unavailable) — METHANOSPHAERA CUNICULI MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'methanosphaera_medium_ii', 'source': 'KOMODO', 'source_id': 'komodo.medium:404', 'original_category': 'archaea'}
pH range
{'min': 7.0, 'max': 7.2}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium404.pdf

Composition

flowchart LR
    CultureMech_001514["methanosphaera_cuniculi_medium"]
    ing0_Fe_NH4_2_SO4_2_x_7_H2O["Fe(NH4)2(SO4)2 x 7 H2O"]
    CultureMech_001514 --> ing0_Fe_NH4_2_SO4_2_x_7_H2O
    CHEBI_131378["CHEBI:131378"]
    ing0_Fe_NH4_2_SO4_2_x_7_H2O -.-> CHEBI_131378
    ing1_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001514 --> ing1_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing1_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing2_Na_acetate_x_3_H2O["Na-acetate x 3 H2O"]
    CultureMech_001514 --> ing2_Na_acetate_x_3_H2O
    CHEBI_32138["CHEBI:32138"]
    ing2_Na_acetate_x_3_H2O -.-> CHEBI_32138
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_001514 --> ing3_Yeast_extract
    ing4_Trypticase_peptone["Trypticase peptone"]
    CultureMech_001514 --> ing4_Trypticase_peptone
    ing5_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001514 --> ing5_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Sodium_resazurin -.-> CHEBI_8806
    ing6_NaHCO3["NaHCO3"]
    CultureMech_001514 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Methanol["Methanol"]
    CultureMech_001514 --> ing7_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing7_Methanol -.-> CHEBI_17790
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001514 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001514 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_K2HPO4["K2HPO4"]
    CultureMech_001514 --> ing10_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing10_K2HPO4 -.-> CHEBI_131527
    ing11_KH2PO4["KH2PO4"]
    CultureMech_001514 --> ing11_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing11_KH2PO4 -.-> CHEBI_63036
    ing12__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001514 --> ing12__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing12__NH4_2SO4 -.-> CHEBI_62946
    ing13_NaCl["NaCl"]
    CultureMech_001514 --> ing13_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing13_NaCl -.-> CHEBI_26710
    ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001514 --> ing14_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001514 --> ing15_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001514 --> ing16_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing17_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001514 --> ing17_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing17_MnSO4_x_H2O -.-> CHEBI_86364
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001514 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001514 --> ing19_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001514 --> ing20_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001514 --> ing21_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing22_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001514 --> ing22_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing22_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing23_H3BO3["H3BO3"]
    CultureMech_001514 --> ing23_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing23_H3BO3 -.-> CHEBI_33118
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001514 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001514 --> ing25_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001514 --> ing26_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing27_Biotin["Biotin"]
    CultureMech_001514 --> ing27_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing27_Biotin -.-> CHEBI_15956
    ing28_Folic_acid["Folic acid"]
    CultureMech_001514 --> ing28_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing28_Folic_acid -.-> CHEBI_27470
    ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001514 --> ing29_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
    CultureMech_001514 --> more["...7 more"]

Ingredients (37)

# Ingredient Concentration Ontology Notes
1 Fe(NH4)2(SO4)2 x 7 H2O
Fe.7H2O.2H4N.2O4S 410.154 g/mol
0.00199203 G_PER_L CHEBI:131378
ferrous ammonium sulfate heptahydrate
2 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.030199202999999997 G_PER_L CHEBI:34887
nickel dichloride
 [Merged 2 duplicates: 0.000199203, 0.03]
3 Na-acetate x 3 H2O
C2H3O2.3H2O.Na 136.079 g/mol
2.98805 G_PER_L CHEBI:32138
sodium acetate trihydrate
4 Yeast extract 0.996016 G_PER_L
5 Trypticase peptone 0.996016 G_PER_L
6 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000498008 G_PER_L CHEBI:8806
Resazurin
7 NaHCO3
CHO3.Na 84.006 g/mol
2.98805 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 Methanol
CH4O 32.042 g/mol
7.88845 G_PER_L CHEBI:17790
methanol
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.298805 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.298805 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 K2HPO4
HO4P.2K 174.174 g/mol
6 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
12 KH2PO4
H2O4P.K 136.084 g/mol
6 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
13 (NH4)2SO4
2H4N.O4S 132.141 g/mol
6 G_PER_L CHEBI:62946
ammonium sulfate
14 NaCl
Cl.Na 58.443 g/mol
13.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 12.0, 1.0]
15 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
5.6 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 2.6, 3.0]
16 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.26 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.16, 0.1]
17 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
18 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
19 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
20 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
21 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
22 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
23 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
24 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
25 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
26 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
27 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
28 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
29 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
30 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
31 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
32 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
33 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
34 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
35 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
36 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
37 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Dissolve ingredients (except bicarbonate, methanol, vitamins, cysteine and sulfide), then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate and adjust pH to 7.0, then distribute under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. After sterilization add methanol (50% v/v), vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Vitamins are sterilized by filtration. Adjust pH of complete medium to 7.0 - 7.2.
  2. After inoculation, pressurize culture bottles with sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure.
  3. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:11.057996Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 404
2026-02-06T05:10:25.341932Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:08:58.339793Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:04.855572Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001514
2026-03-17T06:30:03.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.925170+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOSPHAERA CUNICULI MEDIUM" to "methanosphaera_cuniculi_medium" for consistent matching
2026-05-20T06:01:56.416362+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2}
2026-06-05T07:41:32.562524+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.355737+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:31.300313+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: methanosphaera_cuniculi_medium, methanosphaera_medium_ii
2026-08-06T03:04:31.300381+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint fc691e225bef54539d1135b52f490b8959a02a8206af060aaa724e313b203da8