Overview
- External ontology
- mediadive.medium:408 (source link unavailable) — PSYCHRILYOBACTER SP. MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'py_glutamate_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:408', 'original_category': 'bacterial'}
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium408.pdf
Composition
flowchart LR
CultureMech_001517["psychrilyobacter_sp_medium"]
ing0_Peptone["Peptone"]
CultureMech_001517 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001517 --> ing1_Yeast_extract
ing2_Casamino_acids["Casamino acids"]
CultureMech_001517 --> ing2_Casamino_acids
ing3_L_Glutamic_acid["L-Glutamic acid"]
CultureMech_001517 --> ing3_L_Glutamic_acid
CHEBI_16015["CHEBI:16015"]
ing3_L_Glutamic_acid -.-> CHEBI_16015
ing4_NH4Cl["NH4Cl"]
CultureMech_001517 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_001517 --> ing5_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing6_NaCl["NaCl"]
CultureMech_001517 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_001517 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001517 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001517 --> ing9_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing9_Nitrilotriacetic_acid -.-> CHEBI_44557
ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001517 --> ing10_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
ing11_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001517 --> ing11_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing11_MnSO4_x_H2O -.-> CHEBI_86364
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001517 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001517 --> ing13_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing13_CoSO4_x_7_H2O -.-> CHEBI_91244
ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001517 --> ing14_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001517 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001517 --> ing16_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
ing17_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001517 --> ing17_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing17_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing18_H3BO3["H3BO3"]
CultureMech_001517 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001517 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001517 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001517 --> ing21_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001517 --> ing22_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 10 G_PER_L | ||
| 2 | Yeast extract | 3 G_PER_L | ||
| 3 | Casamino acids | 10 G_PER_L | ||
| 4 |
L-Glutamic acid
C5H9NO4 147.13 g/mol
|
20 G_PER_L |
CHEBI:16015
L-glutamic acid
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
2 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
26.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 25.0, 1.0] |
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 11 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 12 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 15 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 17 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 18 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 22 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 23 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve ingredients (except cysteine), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Add and dissolve cysteine, adjust pH to 7.0 and dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- psychrilyobacter_sp_medium (CultureMech:001517)
- py_glutamate_medium (CultureMech:005228)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.069207Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 408 |
| 2026-01-28T19:48:14.585839Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:52.614113Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:04.887862Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001517 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.960807+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PSYCHRILYOBACTER SP. MEDIUM" to "psychrilyobacter_sp_medium" for consistent matching |
| 2026-06-05T07:41:42.841177+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.912466+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:47:10.515825+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.784057+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: psychrilyobacter_sp_medium, py_glutamate_medium |
| 2026-08-06T03:05:53.784107+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint b911d1ca92f186509317ec1e56d3debd3cce51e0fa0946175fc6a1c2e06f73de |