Overview
- External ontology
- mediadive.medium:429c (source link unavailable) — COLUMBIA-GLUCOSE-CYSTEINE-AGAR
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429c.pdf Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_001536["columbia_glucose_cysteine_agar"]
ing0_Columbia_agar_base["Columbia agar base"]
CultureMech_001536 --> ing0_Columbia_agar_base
ing1_Horse_blood["Horse blood"]
CultureMech_001536 --> ing1_Horse_blood
ing2_Glucose["Glucose"]
CultureMech_001536 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Cysteine["Cysteine"]
CultureMech_001536 --> ing3_Cysteine
CHEBI_15356["CHEBI:15356"]
ing3_Cysteine -.-> CHEBI_15356
ing4_Casein_peptone["Casein peptone"]
CultureMech_001536 --> ing4_Casein_peptone
ing5_Soy_peptone["Soy peptone"]
CultureMech_001536 --> ing5_Soy_peptone
ing6_NaCl["NaCl"]
CultureMech_001536 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Agar["Agar"]
CultureMech_001536 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Calf_brains["Calf brains"]
CultureMech_001536 --> ing8_Calf_brains
ing9_Beef_heart["Beef heart"]
CultureMech_001536 --> ing9_Beef_heart
ing10_Proteose_peptone["Proteose peptone"]
CultureMech_001536 --> ing10_Proteose_peptone
ing11_Dextrose["Dextrose"]
CultureMech_001536 --> ing11_Dextrose
CHEBI_17634["CHEBI:17634"]
ing11_Dextrose -.-> CHEBI_17634
ing12_Sodium_chloride["Sodium chloride"]
CultureMech_001536 --> ing12_Sodium_chloride
ing12_Sodium_chloride -.-> CHEBI_26710
ing13_Disodium_phosphate["Disodium phosphate"]
CultureMech_001536 --> ing13_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing13_Disodium_phosphate -.-> CHEBI_34683
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Columbia agar base | 1000 G_PER_L | ||
| 2 | Horse blood | 40 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
Cysteine
C3H7NO2S 121.161 g/mol
|
1 G_PER_L |
CHEBI:15356
cysteine
|
|
| 5 | Casein peptone | 15 G_PER_L | ||
| 6 | Soy peptone | 5 G_PER_L | ||
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 9 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 10 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 11 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 12 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 13 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 14 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
Preparation
- Mix autoclaved Columbia agar base solution with 4% horse or sheep blood. After cooling to 50°C add 1% glucose and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215.
- Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- columbia_glucose_cysteine_agar (CultureMech:001536)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.110324Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 429c |
| 2026-03-13T06:19:05.028054Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001536 |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.112079+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "COLUMBIA-GLUCOSE-CYSTEINE-AGAR" to "columbia_glucose_cysteine_agar" for consistent matching |
| 2026-06-05T07:40:54.711728+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:29.041609+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d2eef6fb8cbefc73eddfb31a785ce322a8720dc290dbf6c3a6b42d32dd5ca916 |