Overview
- External ontology
- mediadive.medium:440 (source link unavailable) — SKIM MILK-HORNMEAL-MINERAL AGAR
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium440.pdf
Composition
flowchart LR
CultureMech_001549["skim_milk_hornmeal_mineral_agar"]
ing0_Skim_milk["Skim milk"]
CultureMech_001549 --> ing0_Skim_milk
ing1_Bovine_horn_meal["Bovine horn meal"]
CultureMech_001549 --> ing1_Bovine_horn_meal
ing2_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_001549 --> ing2_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing2_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001549 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_K2HPO4["K2HPO4"]
CultureMech_001549 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_NaHCO3["NaHCO3"]
CultureMech_001549 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_FeCl3["FeCl3"]
CultureMech_001549 --> ing6_FeCl3
CHEBI_30808["CHEBI:30808"]
ing6_FeCl3 -.-> CHEBI_30808
ing7_Agar["Agar"]
CultureMech_001549 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Skim milk | 2.5 G_PER_L | ||
| 2 | Bovine horn meal | 10 G_PER_L | ||
| 3 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.5 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.005 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
FeCl3
Cl3Fe 162.204 g/mol
|
0.005 G_PER_L |
CHEBI:30808
iron trichloride
|
|
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Autoclave the skim milk powder separately in 100 ml H2O and add it to the cooled (60°C) agar-medium.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- skim_milk_hornmeal_mineral_agar (CultureMech:001549)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.137555Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 440 |
| 2026-03-13T06:19:05.122551Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001549 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.213182+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SKIM MILK-HORNMEAL-MINERAL AGAR" to "skim_milk_hornmeal_mineral_agar" for consistent matching |
| 2026-06-05T07:41:45.893487+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.506876+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:55.782379+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8c705c36d0291d0c0f30859f14ec5b4cba1fe1114ef6649ebb497aa0f7ea1e09 |