Overview
- External ontology
- mediadive.medium:443 (source link unavailable) — MINERAL MEDIUM WITH PHENYLACETATE
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium443.pdf
Composition
flowchart LR
CultureMech_001552["mineral_medium_with_phenylacetate"]
ing0_Phenyl_acetate["Phenyl acetate"]
CultureMech_001552 --> ing0_Phenyl_acetate
ing1_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_001552 --> ing1_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing1_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing2_KH2PO4["KH2PO4"]
CultureMech_001552 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_001552 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001552 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_001552 --> ing5_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing5_Ferric_ammonium_citrate -.-> CHEBI_31604
ing6_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001552 --> ing6_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing6_MnSO4_x_H2O -.-> CHEBI_86364
ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001552 --> ing7_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing8_CoSO4["CoSO4"]
CultureMech_001552 --> ing8_CoSO4
CHEBI_53470["CHEBI:53470"]
ing8_CoSO4 -.-> CHEBI_53470
ing9_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001552 --> ing9_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing9_Nitrilotriacetic_acid -.-> CHEBI_44557
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Phenyl acetate | 0.5 G_PER_L | ||
| 2 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
9 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
2 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 | Ferric ammonium citrate | 0.005 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 7 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.003 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 8 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.0002 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 9 |
CoSO4
Co.O4S 154.996 g/mol
|
1e-05 G_PER_L |
CHEBI:53470
cobalt(2+) sulfate
|
|
| 10 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.01 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Preparation
- To medium 442 add 0.05% phenylacetate as carbon source instead of toluene, sterilized by filtration.
- Adjust pH to 7.0.
- One drop of toluene is evaporated in a desiccator so that the atmosphere is saturated. 0.1% succinate may be added to the medium as additional carbon source.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mineral_medium_with_phenylacetate (CultureMech:001552)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.145350Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 443 |
| 2026-03-13T06:19:05.147183Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001552 |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.242018+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MINERAL MEDIUM WITH PHENYLACETATE" to "mineral_medium_with_phenylacetate" for consistent matching |
| 2026-06-05T07:41:34.462270+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.003434+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:46.093222+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 120e418d7019341b6f9adaaa0bf30ab1b96f679c9ab669f8c3e89c539b354ce0 |