mineral_medium_with_2_4_dichloro_phenoxyacetic_acid

CultureMech:001555 bacterial DEFINED LIQUID
Original name: MINERAL MEDIUM WITH 2,4-DICHLORO- PHENOXYACETIC ACID

Overview

External ontology
mediadive.medium:446 (source link unavailable) — MINERAL MEDIUM WITH 2,4-DICHLORO- PHENOXYACETIC ACID
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium446.pdf

Composition

flowchart LR
    CultureMech_001555["mineral_medium_with_2_4_dichloro_phenoxyacetic_acid"]
    ing0_2_4_Dichlorophenoxyacetic_acid["2,4-Dichlorophenoxyacetic acid"]
    CultureMech_001555 --> ing0_2_4_Dichlorophenoxyacetic_acid
    ing1_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_001555 --> ing1_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001555 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_001555 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001555 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_001555 --> ing5_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing5_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing6_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001555 --> ing6_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing6_MnSO4_x_H2O -.-> CHEBI_86364
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001555 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing8_CoSO4["CoSO4"]
    CultureMech_001555 --> ing8_CoSO4
    CHEBI_53470["CHEBI:53470"]
    ing8_CoSO4 -.-> CHEBI_53470
    ing9_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001555 --> ing9_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing9_Nitrilotriacetic_acid -.-> CHEBI_44557

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 2,4-Dichlorophenoxyacetic acid 0.5 G_PER_L
2 Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
9 G_PER_L CHEBI:34683
disodium hydrogenphosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
1.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 NH4Cl
Cl.H4N 53.492 g/mol
2 G_PER_L CHEBI:31206
ammonium chloride
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 Ferric ammonium citrate 0.005 G_PER_L CHEBI:31604
ferric ammonium citrate
7 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.003 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
8 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.0002 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
9 CoSO4
Co.O4S 154.996 g/mol
1e-05 G_PER_L CHEBI:53470
cobalt(2+) sulfate
10 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
0.01 G_PER_L CHEBI:44557
nitrilotriacetic acid

Preparation

  1. To medium 442 add 0.05% 2,4-D as carbon source instead of toluene, sterilized by filtration.
  2. Adjust pH to 7.0.
  3. One drop of toluene is evaporated in a desiccator so that the atmosphere is saturated. 0.1% succinate may be added to the medium as additional carbon source.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:11.151473Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 446
2026-03-13T06:19:05.164404Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001555
2026-03-17T06:29:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.262113+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MINERAL MEDIUM WITH 2,4-DICHLORO- PHENOXYACETIC ACID" to "mineral_medium_with_2_4_dichloro_phenoxyacetic_acid" for consistent matching
2026-06-05T07:41:34.247163+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.920010+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:45.868602+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 9927dbf1a0e62441a0acfd1f861c66d7459615c61dc295736bfb06376d801e02