mineral_medium_brunner

CultureMech:001567 bacterial COMPLEX SOLID_AGAR
Original name: MINERAL MEDIUM (BRUNNER)

Overview

External ontology
mediadive.medium:457 (source link unavailable) — MINERAL MEDIUM (BRUNNER)
Applications
  • Microbial cultivation
pH
6.9
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium457.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001567["mineral_medium_brunner"]
    ing0_Na2HPO4["Na2HPO4"]
    CultureMech_001567 --> ing0_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing0_Na2HPO4 -.-> CHEBI_34683
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001567 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001567 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001567 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001567 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_EDTA["EDTA"]
    CultureMech_001567 --> ing5_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing5_EDTA -.-> CHEBI_4735
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001567 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001567 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001567 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_H3BO3["H3BO3"]
    CultureMech_001567 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001567 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001567 --> ing11_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001567 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001567 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_Peptone["Peptone"]
    CultureMech_001567 --> ing14_Peptone
    ing15_Meat_extract["Meat extract"]
    CultureMech_001567 --> ing15_Meat_extract
    ing16_Agar["Agar"]
    CultureMech_001567 --> ing16_Agar
    CHEBI_2509["CHEBI:2509"]
    ing16_Agar -.-> CHEBI_2509
    ing17_Casein_peptone["Casein peptone"]
    CultureMech_001567 --> ing17_Casein_peptone
    ing18_Soy_peptone["Soy peptone"]
    CultureMech_001567 --> ing18_Soy_peptone
    ing19_NaCl["NaCl"]
    CultureMech_001567 --> ing19_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing19_NaCl -.-> CHEBI_26710
    ing20_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_001567 --> ing20_Pancreatic_digest_of_casein
    ing21_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_001567 --> ing21_Peptic_digest_of_soybean_meal
    ing22_Glucose["Glucose"]
    CultureMech_001567 --> ing22_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing22_Glucose -.-> CHEBI_17234
    ing23_Sodium_chloride["Sodium chloride"]
    CultureMech_001567 --> ing23_Sodium_chloride
    ing23_Sodium_chloride -.-> CHEBI_26710
    ing24_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_001567 --> ing24_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing24_Dipotassium_phosphate -.-> CHEBI_131527
    ing25_Agar["Agar"]
    CultureMech_001567 --> ing25_Agar
    ing25_Agar -.-> CHEBI_2509

Ingredients (26)

# Ingredient Concentration Ontology Notes
1 Na2HPO4
HO4P.2Na 141.958 g/mol
2.44 G_PER_L CHEBI:34683
disodium hydrogenphosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
1.52 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 EDTA
C10H16N2O8 292.244 g/mol
0.5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
8 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
9 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
10 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
14 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
15 Peptone 5 G_PER_L
16 Meat extract 3 G_PER_L
17 Agar 50.0 G_PER_L CHEBI:2509
agar
 (for solid medium) [Merged 3 duplicates: 15.0, 15.0, 20.0]
18 Casein peptone 15 G_PER_L
19 Soy peptone 5 G_PER_L
20 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
21 Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
17.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
22 Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
3.0 G_PER_L FOODON:03315720
vegetable protein, hydrolyzed
 Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
23 Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:17234
glucose
 Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
24 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
25 Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
26 Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
15.0 G_PER_L CHEBI:2509
agar
 Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product

Preparation

  1. Adjust pH to 6.9.
  2. Prepare a separate solution of the phosphates and autoclave separately. Combine the two solutions after cooling.
  3. Rehydrate and cultivate lyophilized cells in complex medium (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 457 with the appropriate carbon source.
  4. Adjust pH to 7.0.
  5. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
  6. Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).
  7. pH 7.3
  8. Autoclave at 121°C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:11.172662Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 457
2026-03-13T03:09:01.488792Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:05.230928Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001567
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.338293+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MINERAL MEDIUM (BRUNNER)" to "mineral_medium_brunner" for consistent matching
2026-06-05T07:41:34.083906+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.855598+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:45.579078+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 4f5b47fdd2a3eff1fee9fcc89955c45b5a52d1fdd120546c4bedff8032833dd3