mineral_medium_nagel_and_andreesen

CultureMech:001575 bacterial COMPLEX LIQUID
Original name: MINERAL MEDIUM (NAGEL AND ANDREESEN)

Overview

External ontology
mediadive.medium:461 (source link unavailable) — MINERAL MEDIUM (NAGEL AND ANDREESEN)
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium461.pdf

Composition

flowchart LR
    CultureMech_001575["mineral_medium_nagel_and_andreesen"]
    ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_001575 --> ing0_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001575 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_CaCl2["CaCl2"]
    CultureMech_001575 --> ing2_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing2_CaCl2 -.-> CHEBI_3312
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001575 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_MnSO4["MnSO4"]
    CultureMech_001575 --> ing4_MnSO4
    CHEBI_86360["CHEBI:86360"]
    ing4_MnSO4 -.-> CHEBI_86360
    ing5_NH4Cl["NH4Cl"]
    CultureMech_001575 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_NaCl["NaCl"]
    CultureMech_001575 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Vitamin_B12["Vitamin B12"]
    CultureMech_001575 --> ing7_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing7_Vitamin_B12 -.-> CHEBI_176843
    ing8_Pantothenic_acid["Pantothenic acid"]
    CultureMech_001575 --> ing8_Pantothenic_acid
    CHEBI_7916["CHEBI:7916"]
    ing8_Pantothenic_acid -.-> CHEBI_7916
    ing9_Riboflavin["Riboflavin"]
    CultureMech_001575 --> ing9_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing9_Riboflavin -.-> CHEBI_17015
    ing10_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
    CultureMech_001575 --> ing10_Pyridoxamine_hydrochloride
    CHEBI_131531["CHEBI:131531"]
    ing10_Pyridoxamine_hydrochloride -.-> CHEBI_131531
    ing11_Biotin["Biotin"]
    CultureMech_001575 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_Folic_acid["Folic acid"]
    CultureMech_001575 --> ing12_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing12_Folic_acid -.-> CHEBI_27470
    ing13_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001575 --> ing13_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing13_Nicotinic_acid -.-> CHEBI_15940
    ing14_Nicotine_amide["Nicotine amide"]
    CultureMech_001575 --> ing14_Nicotine_amide
    CHEBI_17154["CHEBI:17154"]
    ing14_Nicotine_amide -.-> CHEBI_17154
    ing15_alpha_lipoic_acid["alpha-lipoic acid"]
    CultureMech_001575 --> ing15_alpha_lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing15_alpha_lipoic_acid -.-> CHEBI_16494
    ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001575 --> ing16_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing17_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001575 --> ing17_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing17_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing18_HCl["HCl"]
    CultureMech_001575 --> ing18_HCl
    CHEBI_17883["CHEBI:17883"]
    ing18_HCl -.-> CHEBI_17883
    ing19_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001575 --> ing19_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing19_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing20_ZnCl2["ZnCl2"]
    CultureMech_001575 --> ing20_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing20_ZnCl2 -.-> CHEBI_49976
    ing21_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001575 --> ing21_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing21_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing22_H3BO3["H3BO3"]
    CultureMech_001575 --> ing22_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing22_H3BO3 -.-> CHEBI_33118
    ing23_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001575 --> ing23_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing23_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing24_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001575 --> ing24_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing24_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001575 --> ing25_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing26_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001575 --> ing26_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing26_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
1.44135 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.248509 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 CaCl2
Ca.2Cl 110.984 g/mol
0.00994036 G_PER_L CHEBI:3312
calcium dichloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.497018 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 MnSO4
Mn.O4S 151.001 g/mol
0.00994036 G_PER_L CHEBI:86360
manganese(II) sulfate
6 NH4Cl
Cl.H4N 53.492 g/mol
0.298211 G_PER_L CHEBI:31206
ammonium chloride
7 NaCl
Cl.Na 58.443 g/mol
0.0497018 G_PER_L CHEBI:26710
sodium chloride
8 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
9 Pantothenic acid
C9H17NO5 219.237 g/mol
0.05 G_PER_L CHEBI:7916
pantothenic acid
10 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
11 Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
0.01 G_PER_L CHEBI:131531
pyridoxamine hydrochloride
12 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
13 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
14 Nicotinic acid
C6H5NO2 123.111 g/mol
0.025 G_PER_L CHEBI:15940
nicotinic acid
15 Nicotine amide
C6H6N2O 122.127 g/mol
0.025 G_PER_L CHEBI:17154
nicotinamide
16 alpha-lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid
17 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
18 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.05 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
19 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
20 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
21 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
22 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
23 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
24 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
25 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
26 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
27 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Dissolve and autoclave the phosphates separately. Add the vitamin solution and trace element solution after autoclaving. After mixing, pH should be 7.5.
  2. Rehydrate and cultivate the lyophilized cells in complex medium recommended for the specific strain (e.g. medium 1, 220 or 535) first. After this reactivation, cultivate on mineral medium 461 with the appropriate carbon source.
  3. Stir for some hours, filter sterilize the solution.
  4. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:11.199468Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 461
2026-03-13T06:19:05.303370Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001575
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.417668+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MINERAL MEDIUM (NAGEL AND ANDREESEN)" to "mineral_medium_nagel_and_andreesen" for consistent matching
2026-06-05T07:41:34.200095+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.900311+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:45.752185+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 4377b672d957aca1650c134bab56998d901ebc9fe5e3b59133f3bb314e9e95c6