Overview
- External ontology
- mediadive.medium:463a (source link unavailable) — EDTA-MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium463a.pdf
Composition
flowchart LR
CultureMech_001582["edta_medium"]
ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_001582 --> ing0_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing1_KH2PO4["KH2PO4"]
CultureMech_001582 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001582 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001582 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_EDTA["EDTA"]
CultureMech_001582 --> ing4_EDTA
CHEBI_4735["CHEBI:4735"]
ing4_EDTA -.-> CHEBI_4735
ing5_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001582 --> ing5_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing5_FeCl2_x_4_H2O -.-> CHEBI_86249
ing6_ZnCl2["ZnCl2"]
CultureMech_001582 --> ing6_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing6_ZnCl2 -.-> CHEBI_49976
ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001582 --> ing7_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
ing8_H3BO3["H3BO3"]
CultureMech_001582 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001582 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001582 --> ing10_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing10_CuCl2_x_2_H2O -.-> CHEBI_86318
ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001582 --> ing11_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001582 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_HCl["HCl"]
CultureMech_001582 --> ing13_HCl
CHEBI_17883["CHEBI:17883"]
ing13_HCl -.-> CHEBI_17883
ing14_Vitamin_B12["Vitamin B12"]
CultureMech_001582 --> ing14_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing14_Vitamin_B12 -.-> CHEBI_176843
ing15_Pantothenic_acid["Pantothenic acid"]
CultureMech_001582 --> ing15_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing15_Pantothenic_acid -.-> CHEBI_7916
ing16_Riboflavin["Riboflavin"]
CultureMech_001582 --> ing16_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing16_Riboflavin -.-> CHEBI_17015
ing17_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
CultureMech_001582 --> ing17_Pyridoxamine_hydrochloride
CHEBI_131531["CHEBI:131531"]
ing17_Pyridoxamine_hydrochloride -.-> CHEBI_131531
ing18_Biotin["Biotin"]
CultureMech_001582 --> ing18_Biotin
CHEBI_15956["CHEBI:15956"]
ing18_Biotin -.-> CHEBI_15956
ing19_Folic_acid["Folic acid"]
CultureMech_001582 --> ing19_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing19_Folic_acid -.-> CHEBI_27470
ing20_Nicotinic_acid["Nicotinic acid"]
CultureMech_001582 --> ing20_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing20_Nicotinic_acid -.-> CHEBI_15940
ing21_Nicotine_amide["Nicotine amide"]
CultureMech_001582 --> ing21_Nicotine_amide
CHEBI_17154["CHEBI:17154"]
ing21_Nicotine_amide -.-> CHEBI_17154
ing22_alpha_lipoic_acid["alpha-lipoic acid"]
CultureMech_001582 --> ing22_alpha_lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing22_alpha_lipoic_acid -.-> CHEBI_16494
ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001582 --> ing23_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
ing24_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001582 --> ing24_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing24_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.41 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.26 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 6 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 7 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.068 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 8 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.062 G_PER_L |
CHEBI:33118
boric acid
|
|
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.12 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 11 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.017 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 12 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.024 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 14 |
HCl
HCl 36.461 g/mol
|
1000 G_PER_L |
CHEBI:17883
hydrogen chloride
|
(0.05 molar) |
| 15 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 16 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
0.05 G_PER_L |
CHEBI:7916
pantothenic acid
|
|
| 17 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 18 |
Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
|
0.01 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
|
| 19 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 20 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 21 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 22 |
Nicotine amide
C6H6N2O 122.127 g/mol
|
0.025 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 23 |
alpha-lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 24 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 25 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.05 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Preparation
- pH 8.0
- Stir for some hours, filter sterilize the solution.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- edta_medium (CultureMech:001582)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.221976Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 463a |
| 2026-03-13T06:19:05.370098Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001582 |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.490570+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "EDTA-MEDIUM" to "edta_medium" for consistent matching |
| 2026-06-05T07:40:59.387167+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.646815+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:33.405155+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 79dce1116b6bff94503a2ae345837a78c0e29a95466168c5574dc93e140b61fb |