mineral_medium_ph_7_25

Overview

External ontology

mediadive.medium:465 (source link unavailable) — MINERAL MEDIUM PH 7.25

Applications

• Microbial cultivation

pH

7.2

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium465.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001585["mineral_medium_ph_7_25"]
    ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_001585 --> ing0_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001585 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001585 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001585 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_001585 --> ing4_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing4_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing5_Peptone["Peptone"]
    CultureMech_001585 --> ing5_Peptone
    ing6_Meat_extract["Meat extract"]
    CultureMech_001585 --> ing6_Meat_extract
    ing7_Agar["Agar"]
    CultureMech_001585 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Casein_peptone["Casein peptone"]
    CultureMech_001585 --> ing8_Casein_peptone
    ing9_Soy_peptone["Soy peptone"]
    CultureMech_001585 --> ing9_Soy_peptone
    ing10_NaCl["NaCl"]
    CultureMech_001585 --> ing10_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing10_NaCl -.-> CHEBI_26710
    ing11_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_001585 --> ing11_Pancreatic_digest_of_casein
    ing12_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_001585 --> ing12_Peptic_digest_of_soybean_meal
    ing13_Glucose["Glucose"]
    CultureMech_001585 --> ing13_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing13_Glucose -.-> CHEBI_17234
    ing14_Sodium_chloride["Sodium chloride"]
    CultureMech_001585 --> ing14_Sodium_chloride
    ing14_Sodium_chloride -.-> CHEBI_26710
    ing15_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_001585 --> ing15_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing15_Dipotassium_phosphate -.-> CHEBI_131527
    ing16_Agar["Agar"]
    CultureMech_001585 --> ing16_Agar
    ing16_Agar -.-> CHEBI_2509
    ing17_Yeast_extract["Yeast extract"]
    CultureMech_001585 --> ing17_Yeast_extract
    ing18_Proteose_peptone["Proteose peptone"]
    CultureMech_001585 --> ing18_Proteose_peptone
    ing19_Casamino_acids["Casamino acids"]
    CultureMech_001585 --> ing19_Casamino_acids
    ing20_Glucose["Glucose"]
    CultureMech_001585 --> ing20_Glucose
    ing20_Glucose -.-> CHEBI_17234
    ing21_Starch["Starch"]
    CultureMech_001585 --> ing21_Starch
    CHEBI_28017["CHEBI:28017"]
    ing21_Starch -.-> CHEBI_28017
    ing22_Na_pyruvate["Na-pyruvate"]
    CultureMech_001585 --> ing22_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing22_Na_pyruvate -.-> CHEBI_50144
    ing23_K2HPO4["K2HPO4"]
    CultureMech_001585 --> ing23_K2HPO4
    ing23_K2HPO4 -.-> CHEBI_131527
    ing24_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001585 --> ing24_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing24_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing25_Na2_EDTA["Na2-EDTA"]
    CultureMech_001585 --> ing25_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing25_Na2_EDTA -.-> CHEBI_64734
    ing26_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001585 --> ing26_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing26_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing27_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001585 --> ing27_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing27_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing28_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001585 --> ing28_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing28_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing29_H3BO3["H3BO3"]
    CultureMech_001585 --> ing29_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing29_H3BO3 -.-> CHEBI_33118
    CultureMech_001585 --> more["...4 more"]

Ingredients (34)

Preparation

1. pH 7.25
2. Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source.
3. Adjust pH to 7.0.
4. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
5. Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).
6. pH 7.3
7. Autoclave at 121°C for 15 min.
8. Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.
9. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• mineral_medium_ph_7_25 (CultureMech:001585)