Overview
- External ontology
- mediadive.medium:465e (source link unavailable) — MINERAL MEDIUM WITH SULFOBENZOIC ACID
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium465e.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_001590["mineral_medium_with_sulfobenzoic_acid"]
ing0_2_Sulfobenzoic_acid["2-Sulfobenzoic acid"]
CultureMech_001590 --> ing0_2_Sulfobenzoic_acid
ing1_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_001590 --> ing1_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing1_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing2_KH2PO4["KH2PO4"]
CultureMech_001590 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_001590 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001590 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_001590 --> ing5_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing5_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing6_p_Aminobenzoate["p-Aminobenzoate"]
CultureMech_001590 --> ing6_p_Aminobenzoate
CHEBI_17836["CHEBI:17836"]
ing6_p_Aminobenzoate -.-> CHEBI_17836
ing7_Biotin["Biotin"]
CultureMech_001590 --> ing7_Biotin
CHEBI_15956["CHEBI:15956"]
ing7_Biotin -.-> CHEBI_15956
ing8_Nicotinic_acid["Nicotinic acid"]
CultureMech_001590 --> ing8_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing8_Nicotinic_acid -.-> CHEBI_15940
ing9_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001590 --> ing9_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing9_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing10_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_001590 --> ing10_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing10_Calcium_pantothenate -.-> CHEBI_31345
ing11_Pyridoxamine["Pyridoxamine"]
CultureMech_001590 --> ing11_Pyridoxamine
CHEBI_16410["CHEBI:16410"]
ing11_Pyridoxamine -.-> CHEBI_16410
ing12_Vitamin_B12["Vitamin B12"]
CultureMech_001590 --> ing12_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing12_Vitamin_B12 -.-> CHEBI_176843
ing13_Na2_EDTA["Na2-EDTA"]
CultureMech_001590 --> ing13_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing13_Na2_EDTA -.-> CHEBI_64734
ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001590 --> ing14_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001590 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001590 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_H3BO3["H3BO3"]
CultureMech_001590 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001590 --> ing18_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001590 --> ing19_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001590 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001590 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_Peptone["Peptone"]
CultureMech_001590 --> ing22_Peptone
ing23_Meat_extract["Meat extract"]
CultureMech_001590 --> ing23_Meat_extract
ing24_Agar["Agar"]
CultureMech_001590 --> ing24_Agar
CHEBI_2509["CHEBI:2509"]
ing24_Agar -.-> CHEBI_2509
ing25_Casein_peptone["Casein peptone"]
CultureMech_001590 --> ing25_Casein_peptone
ing26_Soy_peptone["Soy peptone"]
CultureMech_001590 --> ing26_Soy_peptone
ing27_NaCl["NaCl"]
CultureMech_001590 --> ing27_NaCl
CHEBI_26710["CHEBI:26710"]
ing27_NaCl -.-> CHEBI_26710
ing28_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_001590 --> ing28_Pancreatic_digest_of_casein
ing29_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
CultureMech_001590 --> ing29_Peptic_digest_of_soybean_meal
CultureMech_001590 --> more["...12 more"]
Ingredients (42)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 2-Sulfobenzoic acid | 50 G_PER_L | ||
| 2 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
3.5 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.05 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 7 |
p-Aminobenzoate
C7H6NO2 136.13 g/mol
|
0.01 G_PER_L |
CHEBI:17836
4-aminobenzoate
|
|
| 8 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 9 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.02 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 10 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.01 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 11 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 12 |
Pyridoxamine
C8H12N2O2 168.196 g/mol
|
0.05 G_PER_L |
CHEBI:16410
pyridoxamine
|
|
| 13 | Vitamin B12 | 0.02 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 14 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 15 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 20 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 | Peptone | 5 G_PER_L | ||
| 24 | Meat extract | 3 G_PER_L | ||
| 25 | Agar | 65.0 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) [Merged 4 duplicates: 15.0, 15.0, 20.0, 15.0] |
| 26 | Casein peptone | 15 G_PER_L | ||
| 27 | Soy peptone | 5 G_PER_L | ||
| 28 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 29 |
Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
17.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 30 |
Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
3.0 G_PER_L |
FOODON:03315720
vegetable protein, hydrolyzed
|
Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 31 |
Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 32 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 33 |
Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 34 |
Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
15.0 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 35 | Yeast extract | 0.5 G_PER_L | ||
| 36 | Proteose peptone | 0.5 G_PER_L | ||
| 37 | Casamino acids | 0.5 G_PER_L | ||
| 38 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 39 | Starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
|
| 40 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
0.3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 41 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 42 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Preparation
- Prepare medium 465 supplemented with vitamin solution (see medium 462). Prepare a stock solution of 2-sulfobenzoic acid (20 mg/ml) and sterilize by filtration. Add 50 ml of this stock solution to 1 l of the medium.
- pH 7.25
- Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source.
- First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.
- Adjust pH to 7.0.
- For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
- Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).
- pH 7.3
- Autoclave at 121°C for 15 min.
- Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mineral_medium_with_sulfobenzoic_acid (CultureMech:001590)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.247371Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 465e |
| 2026-01-28T19:48:09.099231Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:41.722857Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:05.451665Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001590 |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.575976+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MINERAL MEDIUM WITH SULFOBENZOIC ACID" to "mineral_medium_with_sulfobenzoic_acid" for consistent matching |
| 2026-06-05T07:41:34.501297+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.436583+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:47:08.014703+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:46.140446+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 30757f2126b4da65ac9def4cd7e419571647d1cf9a34e2378fd54bfe4d14e9cd |