Overview
- External ontology
- mediadive.medium:465g (source link unavailable) — MINERAL MEDIUM WITH CYANURIC ACID AS NITROGEN SOURCE
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium465g.pdf
Composition
flowchart LR
CultureMech_001592["mineral_medium_with_cyanuric_acid_as_nitrogen_source"]
ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_001592 --> ing0_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing1_KH2PO4["KH2PO4"]
CultureMech_001592 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001592 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_Glycerol["Glycerol"]
CultureMech_001592 --> ing3_Glycerol
CHEBI_17754["CHEBI:17754"]
ing3_Glycerol -.-> CHEBI_17754
ing4_Cyanuric_acid["Cyanuric acid"]
CultureMech_001592 --> ing4_Cyanuric_acid
ing5_Vitamin_B12["Vitamin B12"]
CultureMech_001592 --> ing5_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing5_Vitamin_B12 -.-> CHEBI_176843
ing6_Pantothenic_acid["Pantothenic acid"]
CultureMech_001592 --> ing6_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing6_Pantothenic_acid -.-> CHEBI_7916
ing7_Riboflavin["Riboflavin"]
CultureMech_001592 --> ing7_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing7_Riboflavin -.-> CHEBI_17015
ing8_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
CultureMech_001592 --> ing8_Pyridoxamine_hydrochloride
CHEBI_131531["CHEBI:131531"]
ing8_Pyridoxamine_hydrochloride -.-> CHEBI_131531
ing9_Biotin["Biotin"]
CultureMech_001592 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Folic_acid["Folic acid"]
CultureMech_001592 --> ing10_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing10_Folic_acid -.-> CHEBI_27470
ing11_Nicotinic_acid["Nicotinic acid"]
CultureMech_001592 --> ing11_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing11_Nicotinic_acid -.-> CHEBI_15940
ing12_Nicotine_amide["Nicotine amide"]
CultureMech_001592 --> ing12_Nicotine_amide
CHEBI_17154["CHEBI:17154"]
ing12_Nicotine_amide -.-> CHEBI_17154
ing13_alpha_lipoic_acid["alpha-lipoic acid"]
CultureMech_001592 --> ing13_alpha_lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing13_alpha_lipoic_acid -.-> CHEBI_16494
ing14_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001592 --> ing14_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing14_p_Aminobenzoic_acid -.-> CHEBI_30753
ing15_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001592 --> ing15_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing15_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing16_Na2_EDTA["Na2-EDTA"]
CultureMech_001592 --> ing16_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing16_Na2_EDTA -.-> CHEBI_64734
ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001592 --> ing17_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001592 --> ing18_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing19_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001592 --> ing19_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing19_MnCl2_x_4_H2O -.-> CHEBI_86368
ing20_H3BO3["H3BO3"]
CultureMech_001592 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001592 --> ing21_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing21_CoCl2_x_6_H2O -.-> CHEBI_53503
ing22_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001592 --> ing22_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing22_CuCl2_x_2_H2O -.-> CHEBI_86318
ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001592 --> ing23_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001592 --> ing24_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
3.46535 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.990099 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.0990099 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
Glycerol
C3H8O3 92.094 g/mol
|
5.5 G_PER_L |
CHEBI:17754
glycerol
|
|
| 5 | Cyanuric acid | 0.645 G_PER_L | ||
| 6 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 7 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
0.05 G_PER_L |
CHEBI:7916
pantothenic acid
|
|
| 8 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 9 |
Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
|
0.01 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 12 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 13 |
Nicotine amide
C6H6N2O 122.127 g/mol
|
0.025 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 14 |
alpha-lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 15 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 16 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.05 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 17 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 18 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 19 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 20 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 23 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 24 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 25 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source.
- Prepare and autoclave medium. Add 5.5 g/l glycerol from a sterile solution. Add 645 mg/l cyanuric acid from a neutralized and filter sterilized stock solution and 10 ml vitamin solution (see medium 461) to the medium.
- Stir for some hours, filter sterilize the solution.
- First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mineral_medium_with_cyanuric_acid_as_nitrogen_source (CultureMech:001592)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.257474Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 465g |
| 2026-03-13T06:19:05.480168Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001592 |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.606010+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MINERAL MEDIUM WITH CYANURIC ACID AS NITROGEN SOURCE" to "mineral_medium_with_cyanuric_acid_as_nitrogen_source" for consistent matching |
| 2026-06-05T07:41:34.363202+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.962544+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:46.006246+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c9fa8a5c5d1c6c37b7db857693d4e5be04c5b910a94f001780d4bbc5d5b544cd |