mineral_medium_with_atrazin

Overview

External ontology

mediadive.medium:465i (source link unavailable) — MINERAL MEDIUM WITH ATRAZIN

Applications

• Microbial cultivation

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium465i.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001594["mineral_medium_with_atrazin"]
    ing0_2_chloro_4_ethylamino__6__isopropylamino__1_3_5_triazine["2-chloro-4(ethylamino)-6-(isopropylamino)-1,3,5-triazine"]
    CultureMech_001594 --> ing0_2_chloro_4_ethylamino__6__isopropylamino__1_3_5_triazine
    ing1_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_001594 --> ing1_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing1_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001594 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001594 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001594 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_001594 --> ing5_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing5_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing6_Na2_EDTA["Na2-EDTA"]
    CultureMech_001594 --> ing6_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing6_Na2_EDTA -.-> CHEBI_64734
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001594 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001594 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001594 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_H3BO3["H3BO3"]
    CultureMech_001594 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001594 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001594 --> ing12_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001594 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001594 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_Peptone["Peptone"]
    CultureMech_001594 --> ing15_Peptone
    ing16_Meat_extract["Meat extract"]
    CultureMech_001594 --> ing16_Meat_extract
    ing17_Agar["Agar"]
    CultureMech_001594 --> ing17_Agar
    CHEBI_2509["CHEBI:2509"]
    ing17_Agar -.-> CHEBI_2509
    ing18_Casein_peptone["Casein peptone"]
    CultureMech_001594 --> ing18_Casein_peptone
    ing19_Soy_peptone["Soy peptone"]
    CultureMech_001594 --> ing19_Soy_peptone
    ing20_NaCl["NaCl"]
    CultureMech_001594 --> ing20_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing20_NaCl -.-> CHEBI_26710
    ing21_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_001594 --> ing21_Pancreatic_digest_of_casein
    ing22_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_001594 --> ing22_Peptic_digest_of_soybean_meal
    ing23_Glucose["Glucose"]
    CultureMech_001594 --> ing23_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing23_Glucose -.-> CHEBI_17234
    ing24_Sodium_chloride["Sodium chloride"]
    CultureMech_001594 --> ing24_Sodium_chloride
    ing24_Sodium_chloride -.-> CHEBI_26710
    ing25_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_001594 --> ing25_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing25_Dipotassium_phosphate -.-> CHEBI_131527
    ing26_Agar["Agar"]
    CultureMech_001594 --> ing26_Agar
    ing26_Agar -.-> CHEBI_2509
    ing27_Yeast_extract["Yeast extract"]
    CultureMech_001594 --> ing27_Yeast_extract
    ing28_Proteose_peptone["Proteose peptone"]
    CultureMech_001594 --> ing28_Proteose_peptone
    ing29_Casamino_acids["Casamino acids"]
    CultureMech_001594 --> ing29_Casamino_acids
    CultureMech_001594 --> more["...5 more"]

Ingredients (35)

Preparation

1. Suspend atrazine (2-chloro-4(ethylamino)-6-(isopropylamino)-1,3,5-triazine) in methanol (10 mg/ml). Shortly sonicate to reduce particle size. Add stock suspension to medium 465 without nitrogen source (omit ammonium sulphate and calcium nitrate), replace calcium nitrate by calcium chloride) but with 1 g/l glucose as the carbon source. Final concentration of atrazine is 50-100 mg/l.
2. pH 7.25
3. Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source.
4. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.
5. Adjust pH to 7.0.
6. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
7. Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).
8. pH 7.3
9. Autoclave at 121°C for 15 min.
10. Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• mineral_medium_with_atrazin (CultureMech:001594)