alcaligenes_xylosoxydans_medium_with_benzoate

CultureMech:001600 bacterial DEFINED LIQUID
Original name: ALCALIGENES XYLOSOXYDANS MEDIUM WITH BENZOATE

Overview

External ontology
mediadive.medium:471 (source link unavailable) — ALCALIGENES XYLOSOXYDANS MEDIUM WITH BENZOATE
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'medium_471_modified_for_dsm_7136', 'source': 'KOMODO', 'source_id': 'komodo.medium:471_7136', 'original_category': 'bacterial'}
pH
8.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium471.pdf

Composition

flowchart LR
    CultureMech_001600["alcaligenes_xylosoxydans_medium_with_benzoate"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001600 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001600 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001600 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_CaCl2["CaCl2"]
    CultureMech_001600 --> ing3_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing3_CaCl2 -.-> CHEBI_3312
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001600 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_KNO3["KNO3"]
    CultureMech_001600 --> ing5_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing5_KNO3 -.-> CHEBI_63043
    ing6_Na_benzoate["Na-benzoate"]
    CultureMech_001600 --> ing6_Na_benzoate
    CHEBI_113455["CHEBI:113455"]
    ing6_Na_benzoate -.-> CHEBI_113455
    ing7_EDTA["EDTA"]
    CultureMech_001600 --> ing7_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing7_EDTA -.-> CHEBI_4735
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001600 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001600 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001600 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_001600 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001600 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001600 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001600 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001600 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
10.24 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.6 G_PER_L CHEBI:31206
ammonium chloride
4 CaCl2
Ca.2Cl 110.984 g/mol
0.02 G_PER_L CHEBI:3312
calcium dichloride
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 KNO3
K.NO3 101.102 g/mol
4 G_PER_L CHEBI:63043
potassium nitrate
7 Na-benzoate
C7H5O2.Na 144.105 g/mol
1.84 G_PER_L CHEBI:113455
sodium benzoate
8 EDTA
C10H16N2O8 292.244 g/mol
0.5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Autoclave solutions A, B and C separately, combine, adjust pH to 8.2.
  2. Adjust pH to 9.0 with KOH.
  3. First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using a 2 M solution of NaOH; then add ferrous sulfate and the trace element solution SL-6.
  4. First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using a 2 M solution of NaOH; then add ferrous sulfate and the trace element solution SL–6.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:11.280465Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 471
2026-03-13T06:19:05.551517Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001600
2026-03-17T06:29:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.682335+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ALCALIGENES XYLOSOXYDANS MEDIUM WITH BENZOATE" to "alcaligenes_xylosoxydans_medium_with_benzoate" for consistent matching
2026-06-05T07:40:47.654314+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.485535+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:52.716903+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:22.255925+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: alcaligenes_xylosoxydans_medium_with_benzoate, medium_471_modified_for_dsm_7136
2026-08-06T03:05:22.255964+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint ce78ad10ee893061f7c790b29a551d8df18ac2c9b3a1a9e5da71cec9615e06a7