Overview
- External ontology
- mediadive.medium:477 (source link unavailable) — ACIDIHALOBACTER PROSPERUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 2.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium477.pdf
Composition
flowchart LR
CultureMech_001604["acidihalobacter_prosperus_medium"]
ing0_KCl["KCl"]
CultureMech_001604 --> ing0_KCl
CHEBI_32588["CHEBI:32588"]
ing0_KCl -.-> CHEBI_32588
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001604 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001604 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_NH4Cl["NH4Cl"]
CultureMech_001604 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001604 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_K2HPO4["K2HPO4"]
CultureMech_001604 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_KH2PO4["KH2PO4"]
CultureMech_001604 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_NaCl["NaCl"]
CultureMech_001604 --> ing7_NaCl
CHEBI_26710["CHEBI:26710"]
ing7_NaCl -.-> CHEBI_26710
ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001604 --> ing8_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001604 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001604 --> ing10_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
ing11_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001604 --> ing11_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing11_MnSO4_x_H2O -.-> CHEBI_86364
ing12_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001604 --> ing12_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing12_CoSO4_x_7_H2O -.-> CHEBI_91244
ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001604 --> ing13_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing14_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001604 --> ing14_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing14_CuSO4_x_5_H2O -.-> CHEBI_31440
ing15_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001604 --> ing15_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing15_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing16_H3BO3["H3BO3"]
CultureMech_001604 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001604 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001604 --> ing18_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing18_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001604 --> ing19_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KCl
Cl.K 74.551 g/mol
|
0.329341 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.74451 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.44311 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.44311, 3.0] |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.2475 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.23972100000000002 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.139721, 0.1] |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.139721 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.139721 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
NaCl
Cl.Na 58.443 g/mol
|
1.499002 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 0.499002, 1.0] |
| 9 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03199601 G_PER_L |
CHEBI:34887
nickel dichloride
|
[Merged 2 duplicates: 0.00199601, 0.03] |
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
20.060100000000002 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 19.9601, 0.1] |
| 11 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 12 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 13 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 14 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 15 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 16 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 19 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 20 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve ingredients except ferrous sulfate and adjust pH to 2.5 with 4 N H2SO4. Sterilize medium by autoclaving for 15 min at 121°C. Prior to inoculation add FeSO4 x 7 H2O from a 40% (w/v) stock solution prepared with 0.2 N H2SO4 and filter-sterilized under 100% N2 gas atmosphere.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- acidihalobacter_prosperus_medium (CultureMech:001604)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.291376Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 477 |
| 2026-03-13T03:08:38.070837Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:19:05.587227Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001604 |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.720644+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACIDIHALOBACTER PROSPERUS MEDIUM" to "acidihalobacter_prosperus_medium" for consistent matching |
| 2026-06-05T07:40:47.254930+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.577199+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:21.873073+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9b601724caba7d690c5349623cb4b86ee0f20d7df585b7571d5c401f82c56468 |