desulfurella_medium_brackish_water

CultureMech:001606 bacterial COMPLEX LIQUID
Original name: DESULFURELLA MEDIUM (BRACKISH WATER)

Overview

External ontology
mediadive.medium:480a (source link unavailable) — DESULFURELLA MEDIUM (BRACKISH WATER)
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium480a.pdf

Composition

flowchart LR
    CultureMech_001606["desulfurella_medium_brackish_water"]
    ing0_NaCl["NaCl"]
    CultureMech_001606 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001606 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001606 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001606 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_KCl["KCl"]
    CultureMech_001606 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_KH2PO4["KH2PO4"]
    CultureMech_001606 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001606 --> ing6_Yeast_extract
    ing7_Na_acetate["Na-acetate"]
    CultureMech_001606 --> ing7_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing7_Na_acetate -.-> CHEBI_32954
    ing8_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001606 --> ing8_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Sodium_resazurin -.-> CHEBI_8806
    ing9_Sulfur["Sulfur"]
    CultureMech_001606 --> ing9_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing9_Sulfur -.-> CHEBI_26833
    ing10_Na2CO3["Na2CO3"]
    CultureMech_001606 --> ing10_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing10_Na2CO3 -.-> CHEBI_29377
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001606 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_HCl["HCl"]
    CultureMech_001606 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001606 --> ing13_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing14_ZnCl2["ZnCl2"]
    CultureMech_001606 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001606 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_H3BO3["H3BO3"]
    CultureMech_001606 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001606 --> ing17_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001606 --> ing18_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001606 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001606 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing21_Biotin["Biotin"]
    CultureMech_001606 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_Folic_acid["Folic acid"]
    CultureMech_001606 --> ing22_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing22_Folic_acid -.-> CHEBI_27470
    ing23_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001606 --> ing23_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing23_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing24_Thiamine_HCl["Thiamine HCl"]
    CultureMech_001606 --> ing24_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing24_Thiamine_HCl -.-> CHEBI_49105
    ing25_Riboflavin["Riboflavin"]
    CultureMech_001606 --> ing25_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing25_Riboflavin -.-> CHEBI_17015
    ing26_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001606 --> ing26_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing26_Nicotinic_acid -.-> CHEBI_15940
    ing27_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_001606 --> ing27_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing27_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing28_Vitamin_B12["Vitamin B12"]
    CultureMech_001606 --> ing28_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing28_Vitamin_B12 -.-> CHEBI_176843
    ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001606 --> ing29_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
    CultureMech_001606 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
9.98004 G_PER_L CHEBI:26710
sodium chloride
2 NH4Cl
Cl.H4N 53.492 g/mol
0.329341 G_PER_L CHEBI:31206
ammonium chloride
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.329341 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.329341 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 KCl
Cl.K 74.551 g/mol
0.329341 G_PER_L CHEBI:32588
potassium chloride
6 KH2PO4
H2O4P.K 136.084 g/mol
0.329341 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 Yeast extract 0.0998004 G_PER_L
8 Na-acetate
C2H3O2.Na 82.034 g/mol
4.99002 G_PER_L CHEBI:32954
sodium acetate
9 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000499002 G_PER_L CHEBI:8806
Resazurin
10 Sulfur
S 32.067 g/mol
9.98004 G_PER_L CHEBI:26833
sulfur atom
11 Na2CO3
CO3.2Na 105.988 g/mol
0.998004 G_PER_L CHEBI:29377
sodium carbonate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.499002 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
14 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
15 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
17 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
18 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
19 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
22 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
23 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
24 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
25 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
26 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
27 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
28 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
29 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
30 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
31 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Dissolve ingredients (except sulfur, carbonate, vitamins and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 5.9, dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and sterilize medium by heating in a boiling water bath for 2- 3 hours on each of 3 successive days. Add vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.8 - 7.0, if necessary.
  2. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:11.307778Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 480a
2026-02-06T05:10:36.163386Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:05.613839Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001606
2026-03-17T06:30:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.749224+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFURELLA MEDIUM (BRACKISH WATER)" to "desulfurella_medium_brackish_water" for consistent matching
2026-05-20T06:01:15.117077+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0}
2026-06-05T07:40:58.147474+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.167443+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:32.536709+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint d31adfaeef136f327188b89290f1cf305c21194a92444693f82f03d6f23f40a7