hyperthermus_butylicus_medium

CultureMech:001619 archaea COMPLEX LIQUID
Original name: HYPERTHERMUS BUTYLICUS MEDIUM

Overview

External ontology
mediadive.medium:491 (source link unavailable) — HYPERTHERMUS BUTYLICUS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.5, 'max': 7.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium491.pdf

Composition

flowchart LR
    CultureMech_001619["hyperthermus_butylicus_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001619 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001619 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001619 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_KCl["KCl"]
    CultureMech_001619 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NaBr["NaBr"]
    CultureMech_001619 --> ing4_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing4_NaBr -.-> CHEBI_63004
    ing5_H3BO3["H3BO3"]
    CultureMech_001619 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_001619 --> ing6_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing6_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing7__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001619 --> ing7__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing7__NH4_2SO4 -.-> CHEBI_62946
    ing8_NH4Cl["NH4Cl"]
    CultureMech_001619 --> ing8_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing8_NH4Cl -.-> CHEBI_31206
    ing9_Citric_acid["Citric acid"]
    CultureMech_001619 --> ing9_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing9_Citric_acid -.-> CHEBI_30769
    ing10_KI["KI"]
    CultureMech_001619 --> ing10_KI
    CHEBI_8346["CHEBI:8346"]
    ing10_KI -.-> CHEBI_8346
    ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001619 --> ing11_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing12_KH2PO4["KH2PO4"]
    CultureMech_001619 --> ing12_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing12_KH2PO4 -.-> CHEBI_63036
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001619 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_Tryptone["Tryptone"]
    CultureMech_001619 --> ing14_Tryptone
    ing15_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001619 --> ing15_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing15_Sodium_resazurin -.-> CHEBI_8806
    ing16_Sulfur["Sulfur"]
    CultureMech_001619 --> ing16_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing16_Sulfur -.-> CHEBI_26833
    ing17_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001619 --> ing17_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing17_Na2S_x_9_H2O -.-> CHEBI_76209
    ing18_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001619 --> ing18_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing18_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing19_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001619 --> ing19_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing19_MnSO4_x_H2O -.-> CHEBI_86364
    ing20_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001619 --> ing20_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing20_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing21_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001619 --> ing21_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing21_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001619 --> ing22_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing23_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001619 --> ing23_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing23_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing24_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001619 --> ing24_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing24_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001619 --> ing25_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing26_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001619 --> ing26_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing26_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing27_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001619 --> ing27_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing27_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
17.5531 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 16.5531, 1.0]
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.40798 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 3.40798, 3.0]
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.6777 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 KCl
Cl.K 74.551 g/mol
0.321324 G_PER_L CHEBI:32588
potassium chloride
5 NaBr
Br.Na 102.894 g/mol
0.0486855 G_PER_L CHEBI:63004
sodium bromide
6 H3BO3
H3BO3 61.833 g/mol
0.0246056 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.0146056, 0.01]
7 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.00681597 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
8 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.0097371 G_PER_L CHEBI:62946
ammonium sulfate
9 NH4Cl
Cl.H4N 53.492 g/mol
0.486855 G_PER_L CHEBI:31206
ammonium chloride
10 Citric acid
C6H8O7 192.123 g/mol
0.00486855 G_PER_L CHEBI:30769
citric acid
11 KI
I.K 166.002 g/mol
0.00243427 G_PER_L CHEBI:8346
potassium iodide
12 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.830282 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.730282, 0.1]
13 KH2PO4
H2O4P.K 136.084 g/mol
0.486855 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03194742 G_PER_L CHEBI:34887
nickel dichloride
 [Merged 2 duplicates: 0.00194742, 0.03]
15 Tryptone 5.84226 G_PER_L
16 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000486855 G_PER_L CHEBI:8806
Resazurin
17 Sulfur
S 32.067 g/mol
5.84226 G_PER_L CHEBI:26833
sulfur atom
18 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.292113 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
19 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
20 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
21 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
22 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
23 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
24 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
25 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
26 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
27 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
28 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Dissolve ingredients except sulfur and sulfide and adjust pH to 6.0 with sulfuric acid. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, dispense under the same gas atmosphere into anoxic Hungate-type tubes or serum vials containing the appropriate amount of sulfur, only to 30% of their volume to allow for a large headspace. Sterilize medium by heating for 2 - 3 hours in a boiling water bath on each of 3 successive days. Before use reduce the medium by adding sulfide from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere. Check pH and adjust to pH 6.5 - 7.0, if necessary.
  2. After inoculation pressurize culture vessels to 1 bar overpressure with a sterile 80% H2 and 20% CO2 gas mixture.
  3. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:11.354963Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 491
2026-03-13T03:09:27.026432Z data-quality-cleanup-v1.0 Data quality fixes Merged 5 duplicate ingredient(s)
2026-03-13T06:19:05.770728Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001619
2026-03-17T06:30:36.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.916285+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HYPERTHERMUS BUTYLICUS MEDIUM" to "hyperthermus_butylicus_medium" for consistent matching
2026-05-20T06:01:23.006235+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.5-7' -> {'min': 6.5, 'max': 7.0}
2026-06-05T07:41:09.179025+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.978505+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:29.084274+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint f6540234e19e94f163967d03c9498d6c187a8766864fa95a1074d76eb3faec99