Overview
- External ontology
- mediadive.medium:511 (source link unavailable) — METHANOPYRUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium511.pdf
Composition
flowchart LR
CultureMech_001644["methanopyrus_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_001644 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_001644 --> ing1_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing2_KH2PO4["KH2PO4"]
CultureMech_001644 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NaCl["NaCl"]
CultureMech_001644 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001644 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001644 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_Na2SO4["Na2SO4"]
CultureMech_001644 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001644 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_KCl["KCl"]
CultureMech_001644 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_001644 --> ing9_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing9_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing10__NH4_2Ni_SO4_2["(NH4)2Ni(SO4)2"]
CultureMech_001644 --> ing10__NH4_2Ni_SO4_2
CHEBI_86149["CHEBI:86149"]
ing10__NH4_2Ni_SO4_2 -.-> CHEBI_86149
ing11_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001644 --> ing11_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing11_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing12_Sodium_resazurin["Sodium resazurin"]
CultureMech_001644 --> ing12_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing12_Sodium_resazurin -.-> CHEBI_8806
ing13_NaHCO3["NaHCO3"]
CultureMech_001644 --> ing13_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing13_NaHCO3 -.-> CHEBI_32139
ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001644 --> ing14_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing14_Na2S_x_9_H2O -.-> CHEBI_76209
ing15_NaBr["NaBr"]
CultureMech_001644 --> ing15_NaBr
CHEBI_63004["CHEBI:63004"]
ing15_NaBr -.-> CHEBI_63004
ing16_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_001644 --> ing16_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing16_SrCl2_x_6_H2O -.-> CHEBI_36385
ing17_H3BO3["H3BO3"]
CultureMech_001644 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_KI["KI"]
CultureMech_001644 --> ing18_KI
CHEBI_8346["CHEBI:8346"]
ing18_KI -.-> CHEBI_8346
ing19_Na_silicate["Na-silicate"]
CultureMech_001644 --> ing19_Na_silicate
CHEBI_60720["CHEBI:60720"]
ing19_Na_silicate -.-> CHEBI_60720
ing20_NaF["NaF"]
CultureMech_001644 --> ing20_NaF
CHEBI_28741["CHEBI:28741"]
ing20_NaF -.-> CHEBI_28741
ing21_KNO3["KNO3"]
CultureMech_001644 --> ing21_KNO3
CHEBI_63043["CHEBI:63043"]
ing21_KNO3 -.-> CHEBI_63043
ing22_Na2HPO4_x_3_H2O["Na2HPO4 x 3 H2O"]
CultureMech_001644 --> ing22_Na2HPO4_x_3_H2O
CHEBI_34683["CHEBI:34683"]
ing22_Na2HPO4_x_3_H2O -.-> CHEBI_34683
ing23_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001644 --> ing23_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing23_Nitrilotriacetic_acid -.-> CHEBI_44557
ing24_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001644 --> ing24_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing24_MnSO4_x_H2O -.-> CHEBI_86364
ing25_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001644 --> ing25_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing25_FeSO4_x_7_H2O -.-> CHEBI_75836
ing26_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001644 --> ing26_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing26_CoSO4_x_7_H2O -.-> CHEBI_91244
ing27_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001644 --> ing27_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing27_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing28_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001644 --> ing28_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing28_CuSO4_x_5_H2O -.-> CHEBI_31440
ing29_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001644 --> ing29_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing29_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
CultureMech_001644 --> more["...13 more"]
Ingredients (43)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.248262 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
0.0695134 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.0893744 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
12.718 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 11.718, 1.0] |
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
4.73784 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 1.73784, 3.0] |
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.46872 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.804369 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.874578 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.774578, 0.1] |
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.297915 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 10 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.0019861 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 11 |
(NH4)2Ni(SO4)2
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
0.0019861 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 12 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0023861000000000004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
[Merged 2 duplicates: 0.0019861, 0.0004] |
| 13 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000496524 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 14 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.993049 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 15 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.496524 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 16 |
NaBr
Br.Na 102.894 g/mol
|
4 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 17 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
1.8 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
1.31 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 1.3, 0.01] |
| 19 |
KI
I.K 166.002 g/mol
|
0.00125 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 20 |
Na-silicate
2Na.O3Si 122.063 g/mol
|
0.1 G_PER_L |
CHEBI:60720
sodium silicate
|
|
| 21 |
NaF
F.Na 41.988 g/mol
|
0.06 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 22 |
KNO3
K.NO3 101.102 g/mol
|
0.04 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 23 |
Na2HPO4 x 3 H2O
HO4P.2Na 141.958 g/mol
|
0.00025 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 24 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 25 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 26 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 27 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 28 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 29 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 30 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 31 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 32 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 33 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 34 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 35 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 36 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 37 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 38 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 39 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 40 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 41 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 42 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 43 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingregients except bicarbonate, vitamins and sulfide, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into serum vials to 30% of their volume and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins should be sterilized by filtration. Adjust pH of the complete medium to 6.5, if necessary.
- After inoculation pressurize vessels to 2 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methanopyrus_medium (CultureMech:001644)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.468976Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 511 |
| 2026-01-28T19:48:42.947169Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-06T05:10:55.388621Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:44.264987Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:19:06.129135Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001644 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.294538+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOPYRUS MEDIUM" to "methanopyrus_medium" for consistent matching |
| 2026-06-05T07:41:32.104492+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 39 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.469652+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:07.187471+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 9 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:30.927794+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b882b2e7743b2a2cbbd9c62e8ef23f75f8ca7d235172142238150c6f239fe8d6 |