clostridium_viride_medium

Overview

External ontology

mediadive.medium:526 (source link unavailable) — CLOSTRIDIUM VIRIDE MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 7.6, 'max': 7.8}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium526.pdf

Composition

flowchart LR
    CultureMech_001657["clostridium_viride_medium"]
    ing0_5_Aminovaleric_acid["5-Aminovaleric acid"]
    CultureMech_001657 --> ing0_5_Aminovaleric_acid
    CHEBI_15887["CHEBI:15887"]
    ing0_5_Aminovaleric_acid -.-> CHEBI_15887
    ing1_K2HPO4["K2HPO4"]
    CultureMech_001657 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_001657 --> ing2_Yeast_extract
    ing3_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001657 --> ing3_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Sodium_resazurin -.-> CHEBI_8806
    ing4_NaHCO3["NaHCO3"]
    CultureMech_001657 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001657 --> ing5_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing6_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001657 --> ing6_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing6_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001657 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001657 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001657 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001657 --> ing10_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001657 --> ing11_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing12_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001657 --> ing12_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing12_MnSO4_x_H2O -.-> CHEBI_86364
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001657 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_VOSO4_x_5_H2O["VOSO4 x 5 H2O"]
    CultureMech_001657 --> ing14_VOSO4_x_5_H2O
    CHEBI_132758["CHEBI:132758"]
    ing14_VOSO4_x_5_H2O -.-> CHEBI_132758
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001657 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001657 --> ing16_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing16_CuSO4_x_5_H2O -.-> CHEBI_31440

Ingredients (17)

Preparation

1. Dissolve ingredients (except bicarbonate and cysteine), adjust pH to 7.0 with NaOH and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min. Add and dissolve solid bicarbonate and cysteine, dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving adjust pH of medium to 7.6 - 7.8 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere.
2. Dissolve mineral salts in 700 ml water acidified with 2 ml of conc. HCl. Separately dissolve 5.00 g of NTA in 300 ml water add 10 N NaOH until all the NTA has dissolved and the pH is 7.0. While stirring, slowly add the NTA solution to the salt solution. Stir until the solution is almost clear. Store cool and dark.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• clostridium_viride_medium (CultureMech:001657)