heliobacillus_mobilis_medium_modified_van_niels_medium

CultureMech:001669 bacterial COMPLEX LIQUID
Original name: HELIOBACILLUS MOBILIS MEDIUM (modified van Niel's medium)

Overview

External ontology
mediadive.medium:537 (source link unavailable) — HELIOBACILLUS MOBILIS MEDIUM (modified van Niel's medium)
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'heliobacillus_mobilis_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:537', 'original_category': 'bacterial'}
pH
6.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium537.pdf

Composition

flowchart LR
    CultureMech_001669["heliobacillus_mobilis_medium_modified_van_niels_medium"]
    ing0_MgSO4["MgSO4"]
    CultureMech_001669 --> ing0_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing0_MgSO4 -.-> CHEBI_32599
    ing1_EDTA["EDTA"]
    CultureMech_001669 --> ing1_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing1_EDTA -.-> CHEBI_4735
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_001669 --> ing2_Yeast_extract
    ing3_K2HPO4["K2HPO4"]
    CultureMech_001669 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Na_pyruvate["Na-pyruvate"]
    CultureMech_001669 --> ing4_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing4_Na_pyruvate -.-> CHEBI_50144
    ing5_H3BO3["H3BO3"]
    CultureMech_001669 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001669 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001669 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001669 --> ing8_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001669 --> ing9_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing9_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing10_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001669 --> ing10_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing10_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing11_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_001669 --> ing11_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing11_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001669 --> ing12_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing12_CaCl2_x_2_H2O -.-> CHEBI_86158

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 MgSO4
Mg.O4S 120.368 g/mol
0.1 G_PER_L CHEBI:32599
magnesium sulfate
2 EDTA
C10H16N2O8 292.244 g/mol
0.002 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
3 Yeast extract 10 G_PER_L
4 K2HPO4
HO4P.2K 174.174 g/mol
5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 Na-pyruvate
C3H3O3.Na 110.044 g/mol
100 G_PER_L CHEBI:50144
sodium pyruvate
6 H3BO3
H3BO3 61.833 g/mol
2.86 G_PER_L CHEBI:33118
boric acid
7 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1.81 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
8 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.222 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
9 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.39 G_PER_L CHEBI:75213
sodium molybdate dihydrate
10 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.079 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
11 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0494 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
12 Ferric ammonium citrate 2 G_PER_L CHEBI:31604
ferric ammonium citrate
13 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3 G_PER_L CHEBI:86158
calcium chloride dihydrate

Preparation

  1. Adjust pH to 6.8. Boil the medium for few minutes. Fill in tubes with rubber stoppers under nitrogen gas. Fill immediately about 45 ml Sterilize at 121°C for 15 min. Cool to room temperature and aseptically add the following filter-sterilized solutions.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:11.561991Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 537
2026-03-13T06:19:06.333968Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001669
2026-03-17T06:30:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.519739+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HELIOBACILLUS MOBILIS MEDIUM (modified van Niel's medium)" to "heliobacillus_mobilis_medium_modified_van_niels_medium" for consistent matching
2026-06-05T07:41:08.361384+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.713715+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:37.461363+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: heliobacillus_mobilis_medium, heliobacillus_mobilis_medium_modified_van_niels_medium
2026-08-06T03:05:37.461398+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 81257360b6b98e6457f21c77619e9093db41cfa37e0d0db1c3cc6ca97647a26f