Overview
- External ontology
- mediadive.medium:539 (source link unavailable) — RUMINICLOSTRIDIUM CELLOBIOPARUM MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'clostridium_termitidis_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:539', 'original_category': 'bacterial'}
- {'name': 'clostridium_termitidis_medium_replace_cellulose_with_cellobiose', 'source': 'KOMODO', 'source_id': 'komodo.medium:539_replace_Cellulose_with_Cellobiose', 'original_category': 'bacterial'}
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium539.pdf
Composition
flowchart LR
CultureMech_001671["ruminiclostridium_cellobioparum_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001671 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NH4Cl["NH4Cl"]
CultureMech_001671 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_NaCl["NaCl"]
CultureMech_001671 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001671 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_KCl["KCl"]
CultureMech_001671 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001671 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Yeast_extract["Yeast extract"]
CultureMech_001671 --> ing6_Yeast_extract
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_001671 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_Na2CO3["Na2CO3"]
CultureMech_001671 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_Cellobiose["Cellobiose"]
CultureMech_001671 --> ing9_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing9_Cellobiose -.-> CHEBI_17057
ing10_Cellulose["Cellulose"]
CultureMech_001671 --> ing10_Cellulose
CHEBI_18246["CHEBI:18246"]
ing10_Cellulose -.-> CHEBI_18246
ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001671 --> ing11_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing11_Na2S_x_9_H2O -.-> CHEBI_76209
ing12_HCl["HCl"]
CultureMech_001671 --> ing12_HCl
CHEBI_17883["CHEBI:17883"]
ing12_HCl -.-> CHEBI_17883
ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001671 --> ing13_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
ing14_ZnCl2["ZnCl2"]
CultureMech_001671 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001671 --> ing15_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
ing16_H3BO3["H3BO3"]
CultureMech_001671 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001671 --> ing17_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001671 --> ing18_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001671 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001671 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1998 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.2997 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.999001 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.3996 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.499501 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.14985 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 | Yeast extract | 0.499501 G_PER_L | ||
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0004995 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.999001 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 |
Cellobiose
C12H22O11 342.297 g/mol
|
4.99501 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 11 |
Cellulose
(C6H10O5)n.H2O 180.156 g/mol
|
4.99501 G_PER_L |
CHEBI:18246
(1->4)-beta-D-glucan
|
Optional ingredient (MN 301) |
| 12 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.3996 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 13 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 14 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 16 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 19 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Dissolve ingredients (except carbonate, cellobiose and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add cellobiose (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.0, if necessary.
- Note: Some strains can be adapted to cellulose as substrate using 5.00 g/l cellulose powder MN 301 (MACHEREY-NAGEL).
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- clostridium_termitidis_medium (CultureMech:005966)
- clostridium_termitidis_medium_replace_cellulose_with_cellobiose (CultureMech:005965)
- ruminiclostridium_cellobioparum_medium (CultureMech:001671)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.565989Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 539 |
| 2026-03-13T06:19:06.349568Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001671 |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.535785+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RUMINICLOSTRIDIUM CELLOBIOPARUM MEDIUM" to "ruminiclostridium_cellobioparum_medium" for consistent matching |
| 2026-06-05T07:41:44.812482+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.134239+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:28.579934+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: clostridium_termitidis_medium, clostridium_termitidis_medium_replace_cellulose_with_cellobiose, ruminiclostridium_cellobioparum_medium |
| 2026-08-06T03:05:28.579994+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 6b4a930d04da4e502e746696d57d89c0dfd9827ead2123ba52faec8b63823aa8 |