cm_ye_medium

CultureMech:001683 bacterial COMPLEX SOLID_AGAR
Original name: CM + YE MEDIUM

Overview

External ontology
mediadive.medium:549 (source link unavailable) — CM + YE MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'medium_549_modified_for_dsm_25790', 'source': 'KOMODO', 'source_id': 'komodo.medium:549_25790', 'original_category': 'bacterial'}
  • {'name': 'medium_549_modified_for_dsm_44640', 'source': 'KOMODO', 'source_id': 'komodo.medium:549_44640', 'original_category': 'bacterial'}
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium549.pdf

Composition

flowchart LR
    CultureMech_001683["cm_ye_medium"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_001683 --> ing0_Casamino_acids
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001683 --> ing1_Yeast_extract
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001683 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_Na3_citrate["Na3-citrate"]
    CultureMech_001683 --> ing3_Na3_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Na3_citrate -.-> CHEBI_53258
    ing4_KCl["KCl"]
    CultureMech_001683 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NaCl["NaCl"]
    CultureMech_001683 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Agar["Agar"]
    CultureMech_001683 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001683 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_HCl["HCl"]
    CultureMech_001683 --> ing8_HCl
    CHEBI_17883["CHEBI:17883"]
    ing8_HCl -.-> CHEBI_17883

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Casamino acids 7.5 G_PER_L
2 Yeast extract 10 G_PER_L
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 Na3-citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
5 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
6 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
7 Agar 15 G_PER_L CHEBI:2509
agar
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
10 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 HCl
HCl 36.461 g/mol
0.03646 G_PER_L CHEBI:17883
hydrogen chloride

Preparation

  1. pH 7.4 (NaOH)
  2. Prepare 4.98% FeSO4 x 7 H2O (= 10 g Fe in 1l 0.001 M HCl)

Target organisms

No target organisms are recorded.

Provenance

Merged from 3 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:11.589060Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 549
2026-01-28T19:48:28.711109Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:06.418578Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001683
2026-03-17T06:30:26.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.611604+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CM + YE MEDIUM" to "cm_ye_medium" for consistent matching
2026-06-05T07:40:54.538262+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:36.401828+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-08-06T03:05:28.911248+00:00 recipe-merger Merged 3 duplicate recipes into canonical record Sources: cm_ye_medium, medium_549_modified_for_dsm_25790, medium_549_modified_for_dsm_44640
2026-08-06T03:05:28.911289+00:00 merge_recipes.py MERGED_RECIPES merged 3 source recipe(s) on fingerprint 86792d506dfc9417bb5cf38da41ae9c002111a25258266cb0666cacd47651e53