Overview
- External ontology
- mediadive.medium:54a (source link unavailable) — REACTIVATION WITH LIQUID MEDIUM 545
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium54a.pdf
Composition
flowchart LR
CultureMech_001684["reactivation_with_liquid_medium_545"]
ing0_Glucose["Glucose"]
CultureMech_001684 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Yeast_extract["Yeast extract"]
CultureMech_001684 --> ing1_Yeast_extract
ing2_CaCO3["CaCO3"]
CultureMech_001684 --> ing2_CaCO3
CHEBI_3311["CHEBI:3311"]
ing2_CaCO3 -.-> CHEBI_3311
ing3_Agar["Agar"]
CultureMech_001684 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Casein_peptone["Casein peptone"]
CultureMech_001684 --> ing4_Casein_peptone
ing5_Soy_peptone["Soy peptone"]
CultureMech_001684 --> ing5_Soy_peptone
ing6_D____Glucose["D(+)-Glucose"]
CultureMech_001684 --> ing6_D____Glucose
CHEBI_17634["CHEBI:17634"]
ing6_D____Glucose -.-> CHEBI_17634
ing7_NaCl["NaCl"]
CultureMech_001684 --> ing7_NaCl
CHEBI_26710["CHEBI:26710"]
ing7_NaCl -.-> CHEBI_26710
ing8_K2HPO4["K2HPO4"]
CultureMech_001684 --> ing8_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4 -.-> CHEBI_131527
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Yeast extract | 10 G_PER_L | ||
| 3 |
CaCO3
CO3.Ca 100.086 g/mol
|
20 G_PER_L |
CHEBI:3311
calcium carbonate
|
|
| 4 | Agar | 17 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 5 | Casein peptone | 17 G_PER_L | ||
| 6 | Soy peptone | 3 G_PER_L | ||
| 7 |
D(+)-Glucose
C6H12O6 180.156 g/mol
|
2.5 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 8 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 9 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Preparation
- Rehydrate and grow lyophilized cells from the ampoule in liquid medium 54 or 545. Subsequent subculturing may be carried out in liquid medium or on agar plates medium 54.
- Adjust pH to 7.3.
- The same as Caso Bouillon (Merck or Oxoid, Germany).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- reactivation_with_liquid_medium_545 (CultureMech:001684)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.055336Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 54a |
| 2026-03-13T06:19:06.423980Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001684 |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.617188+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "REACTIVATION WITH LIQUID MEDIUM 545" to "reactivation_with_liquid_medium_545" for consistent matching |
| 2026-06-05T07:41:44.106110+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.879194+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.520706+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint de9f4b372d31d4789430cfa4f54126310ac0bbdd80031930576a5a1939189bfb |