Overview
- External ontology
- mediadive.medium:558a (source link unavailable) — CLOSTRIDIUM (RB-9) MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'clostridium_rb_1_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:558', 'original_category': 'bacterial'}
- {'name': 'rb_1_rb_9_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:558', 'original_category': 'bacterial'}
- {'name': 'rb_1_rb_9_medium_replace_glucose_with_starch', 'source': 'KOMODO', 'source_id': 'komodo.medium:558_replace_Glucose_with_Starch', 'original_category': 'bacterial'}
- pH range
- {'min': 5.0, 'max': 6.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium558a.pdf
Composition
flowchart LR
CultureMech_001693["clostridium_rb_9_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001693 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_001693 --> ing1_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing1_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing2_NH4Cl["NH4Cl"]
CultureMech_001693 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001693 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_Na2SO4["Na2SO4"]
CultureMech_001693 --> ing4_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing4_Na2SO4 -.-> CHEBI_32149
ing5_Sodium_resazurin["Sodium resazurin"]
CultureMech_001693 --> ing5_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Sodium_resazurin -.-> CHEBI_8806
ing6_D_Glucose["D-Glucose"]
CultureMech_001693 --> ing6_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing6_D_Glucose -.-> CHEBI_17634
ing7_Yeast_extract["Yeast extract"]
CultureMech_001693 --> ing7_Yeast_extract
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001693 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001693 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001693 --> ing10_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001693 --> ing11_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
ing12_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001693 --> ing12_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing12_MnSO4_x_H2O -.-> CHEBI_86364
ing13_NaCl["NaCl"]
CultureMech_001693 --> ing13_NaCl
CHEBI_26710["CHEBI:26710"]
ing13_NaCl -.-> CHEBI_26710
ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001693 --> ing14_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001693 --> ing15_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001693 --> ing16_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001693 --> ing17_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001693 --> ing18_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
ing19_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001693 --> ing19_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing19_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing20_H3BO3["H3BO3"]
CultureMech_001693 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001693 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001693 --> ing22_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001693 --> ing23_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001693 --> ing24_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing25_Biotin["Biotin"]
CultureMech_001693 --> ing25_Biotin
CHEBI_15956["CHEBI:15956"]
ing25_Biotin -.-> CHEBI_15956
ing26_Folic_acid["Folic acid"]
CultureMech_001693 --> ing26_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing26_Folic_acid -.-> CHEBI_27470
ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001693 --> ing27_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing28_Thiamine_HCl["Thiamine HCl"]
CultureMech_001693 --> ing28_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing28_Thiamine_HCl -.-> CHEBI_49105
ing29_Riboflavin["Riboflavin"]
CultureMech_001693 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_001693 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.48368 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
4.1543 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.49456 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.356083 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.98912 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 6 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.00049456 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
D-Glucose
C6H12O6 180.156 g/mol
|
9.8912 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 8 | Yeast extract | 1.97824 G_PER_L | ||
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.49456 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.49456 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 12 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 13 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 14 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 15 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 16 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 17 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 18 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 19 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 20 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 24 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 25 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 26 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 27 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 28 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 29 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 31 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 32 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 33 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 34 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 35 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except glucose, yeast extract, vitamin solution and reducing agents), adjust pH to 5 - 6 using HCl and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add glucose, yeast extract, vitamin solution, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Adjust pH of complete medium to 5.0 - 6.0, if necessary.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 4 source records.
- clostridium_rb_1_medium (CultureMech:001692)
- clostridium_rb_9_medium (CultureMech:001693)
- rb_1_rb_9_medium (CultureMech:006009)
- rb_1_rb_9_medium_replace_glucose_with_starch (CultureMech:006008)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.607777Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 558a |
| 2026-02-06T05:10:26.471505Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:06.487720Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001693 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.688352+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CLOSTRIDIUM (RB-9) MEDIUM" to "clostridium_rb_9_medium" for consistent matching |
| 2026-05-20T06:01:12.042288+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '5-6' -> {'min': 5.0, 'max': 6.0} |
| 2026-06-05T07:40:54.186634+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.794506+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:28.528549+00:00 | recipe-merger | Merged 4 duplicate recipes into canonical record | Sources: clostridium_rb_1_medium, clostridium_rb_9_medium, rb_1_rb_9_medium, rb_1_rb_9_medium_replace_glucose_with_starch |
| 2026-08-06T03:05:28.528653+00:00 | merge_recipes.py | MERGED_RECIPES | merged 4 source recipe(s) on fingerprint 38eb7f95424a87c108e267a88665ffc874ba207bc76150041943d7c2a8c137d9 |