Overview
- External ontology
- mediadive.medium:576 (source link unavailable) — SUCCINATE MINERAL MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium576.pdf
Composition
flowchart LR
CultureMech_001703["succinate_mineral_medium"]
ing0_Succinate["Succinate"]
CultureMech_001703 --> ing0_Succinate
CHEBI_26806["CHEBI:26806"]
ing0_Succinate -.-> CHEBI_26806
ing1_KH2PO4["KH2PO4"]
CultureMech_001703 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_001703 --> ing2_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001703 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_NH4Cl["NH4Cl"]
CultureMech_001703 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001703 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Na2_EDTA["Na2-EDTA"]
CultureMech_001703 --> ing6_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing6_Na2_EDTA -.-> CHEBI_64734
ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001703 --> ing7_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
ing8_CoCl2_x_2_H2O["CoCl2 x 2 H2O"]
CultureMech_001703 --> ing8_CoCl2_x_2_H2O
CHEBI_35696["CHEBI:35696"]
ing8_CoCl2_x_2_H2O -.-> CHEBI_35696
ing9_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001703 --> ing9_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing9_CuCl2_x_2_H2O -.-> CHEBI_86318
ing10_H3BO3["H3BO3"]
CultureMech_001703 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001703 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001703 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001703 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_ZnCl2["ZnCl2"]
CultureMech_001703 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Succinate | 1.1607 G_PER_L |
CHEBI:26806
succinate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.39 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
1.63 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.062 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.02 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.14 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
[Merged 2 duplicates: 0.25, 0.25] |
| 8 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.14 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 9 |
CoCl2 x 2 H2O
2Cl.Co 129.839 g/mol
|
0.028 G_PER_L |
CHEBI:35696
cobalt dichloride
|
|
| 10 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.0007 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.004 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.003 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.00165 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.005 G_PER_L |
CHEBI:49976
zinc dichloride
|
Preparation
- pH 6.8
- Autoclave trace element solutions A and B separately and mix identical volumes:
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- succinate_mineral_medium (CultureMech:001703)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.633276Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 576 |
| 2026-03-13T03:08:59.933390Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:06.565508Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001703 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.773514+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SUCCINATE MINERAL MEDIUM" to "succinate_mineral_medium" for consistent matching |
| 2026-06-05T07:41:47.194373+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.916131+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:56.636967+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8c849008eb6d63b0dc8655f9ea3997a651fdcd8f28256ab2d5dd0e06f35357b0 |