bcye_agar

Overview

External ontology

mediadive.medium:585 (source link unavailable) — BCYE AGAR

Applications

• Microbial cultivation

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium585.pdf

Composition

flowchart LR
    CultureMech_001711["bcye_agar"]
    ing0_OXOID_Legionella_CYE_Agar_base["OXOID Legionella CYE-Agar base"]
    CultureMech_001711 --> ing0_OXOID_Legionella_CYE_Agar_base
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001711 --> ing1_Yeast_extract
    ing2_ACES["ACES"]
    CultureMech_001711 --> ing2_ACES
    CHEBI_39061["CHEBI:39061"]
    ing2_ACES -.-> CHEBI_39061
    ing3_Activated_charcoal["Activated charcoal"]
    CultureMech_001711 --> ing3_Activated_charcoal
    ing4_Agar["Agar"]
    CultureMech_001711 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001711 --> ing5_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing6_Fe4_PO4_2["Fe4(PO4)2"]
    CultureMech_001711 --> ing6_Fe4_PO4_2
    CHEBI_132767["CHEBI:132767"]
    ing6_Fe4_PO4_2 -.-> CHEBI_132767

Ingredients (7)

Preparation

1. Dehydrated media preparations are available from media distributors.
2. We use OXOID Legionella CYE-Agar base with Legionella BCYE supplement.
3. For solubilization of the lyophilized pellet use standard liquid media. Although Legionellae do not grow in those media they are suitable for resuspension and transfer onto agar plates.
4. Note: Alternatively use CYE-ACES AGAR
5. Add Cysteine-HCl, then ferric pyrophosphate solution to solution A. Adjust the complete medium to pH 6.9 ± 0.5 at 50°C with sterile 1.0 N KOH or HCl. The pH of the medium is critical. Finally, add solution B. Swirl medium in flask during dispensing to petri dishes or tubes in order to keep charcoal suspended.
6. Dissolve ACES and bring to pH 6.9 by slowly adding 1 N KOH. Add the other components and dissolve by boiling. Autoclave at 121°C for 15 minutes. Cool to 50 - 55°C.
7. Autoclave at 121°C and cool to 50 - 55°C.
8. Prepare a fresh solution and filter-sterilize it.
9. Prepare a fresh solution by heating to 50 - 55°C in a water-bath. Filter-sterilize solution separately.
10. Please note: Soluble ferric pyrophosphate must be kept dry and in the dark. Do not use if chemical looses its green colour and becomes brown or yellow.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• bcye_agar (CultureMech:001711)