Overview
- External ontology
- mediadive.medium:58 (source link unavailable) — BIFIDOBACTERIUM MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_58_modified_for_dsm_16839', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_16839', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_16840', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_16840', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_16980', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_16980', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_16981', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_16981', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_17042', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_17042', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_17774', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_17774', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_17775', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_17775', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_17776', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_17776', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_17777', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_17777', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_17778', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_17778', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_17779', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_17779', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_20461', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_20461', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_20462', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_20462', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_20465', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_20465', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_20466', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_20466', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_20467', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_20467', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_20756', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_20756', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_20757', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_20757', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_20762', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_20762', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_20764', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_20764', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_20765', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_20765', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_21655', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_21655', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_24661', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_24661', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_7198', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_7198', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_7199', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_7199', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_7200', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_7200', 'original_category': 'bacterial'}
- {'name': 'medium_58_modified_for_dsm_7201', 'source': 'KOMODO', 'source_id': 'komodo.medium:58_7201', 'original_category': 'bacterial'}
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium58.pdf
Composition
flowchart LR
CultureMech_001717["bifidobacterium_medium"]
ing0_Casein_peptone["Casein peptone"]
CultureMech_001717 --> ing0_Casein_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001717 --> ing1_Yeast_extract
ing2_Meat_extract["Meat extract"]
CultureMech_001717 --> ing2_Meat_extract
ing3_Bacto_Soytone["Bacto Soytone"]
CultureMech_001717 --> ing3_Bacto_Soytone
ing4_Glucose["Glucose"]
CultureMech_001717 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_K2HPO4["K2HPO4"]
CultureMech_001717 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001717 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001717 --> ing7_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing7_MnSO4_x_H2O -.-> CHEBI_86364
ing8_Tween_80["Tween 80"]
CultureMech_001717 --> ing8_Tween_80
CHEBI_53426["CHEBI:53426"]
ing8_Tween_80 -.-> CHEBI_53426
ing9_NaCl["NaCl"]
CultureMech_001717 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001717 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing11_Resazurin["Resazurin"]
CultureMech_001717 --> ing11_Resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Resazurin -.-> CHEBI_8806
ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001717 --> ing12_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
ing13_KH2PO4["KH2PO4"]
CultureMech_001717 --> ing13_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing13_KH2PO4 -.-> CHEBI_63036
ing14_NaHCO3["NaHCO3"]
CultureMech_001717 --> ing14_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing14_NaHCO3 -.-> CHEBI_32139
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein peptone | 10 G_PER_L | ||
| 2 | Yeast extract | 5 G_PER_L | ||
| 3 | Meat extract | 5 G_PER_L | ||
| 4 | Bacto Soytone | 5 G_PER_L | ||
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3.0 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
[Merged 2 duplicates: 2.0, 1.0] |
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.2, 0.5] |
| 8 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.05 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 9 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
7.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 5.0, 2.0] |
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 12 |
Resazurin
C12H7NO4 229.191 g/mol
|
4 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 13 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 14 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 15 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Preparation
- The cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 6.8 using 8 N NaOH. Distribute under N2 and autoclave.
Target organisms
No target organisms are recorded.
Provenance
Merged from 28 source records.
- bifidobacterium_medium (CultureMech:001717)
- medium_58_modified_for_dsm_16839 (CultureMech:006025)
- medium_58_modified_for_dsm_16840 (CultureMech:006026)
- medium_58_modified_for_dsm_16980 (CultureMech:006027)
- medium_58_modified_for_dsm_16981 (CultureMech:006028)
- medium_58_modified_for_dsm_17042 (CultureMech:006029)
- medium_58_modified_for_dsm_17774 (CultureMech:006030)
- medium_58_modified_for_dsm_17775 (CultureMech:006031)
- medium_58_modified_for_dsm_17776 (CultureMech:006032)
- medium_58_modified_for_dsm_17777 (CultureMech:006033)
- medium_58_modified_for_dsm_17778 (CultureMech:006034)
- medium_58_modified_for_dsm_17779 (CultureMech:006035)
- medium_58_modified_for_dsm_20461 (CultureMech:006036)
- medium_58_modified_for_dsm_20462 (CultureMech:006037)
- medium_58_modified_for_dsm_20465 (CultureMech:006038)
- medium_58_modified_for_dsm_20466 (CultureMech:006039)
- medium_58_modified_for_dsm_20467 (CultureMech:006040)
- medium_58_modified_for_dsm_20756 (CultureMech:006041)
- medium_58_modified_for_dsm_20757 (CultureMech:006042)
- medium_58_modified_for_dsm_20762 (CultureMech:006043)
- medium_58_modified_for_dsm_20764 (CultureMech:006044)
- medium_58_modified_for_dsm_20765 (CultureMech:006045)
- medium_58_modified_for_dsm_21655 (CultureMech:006046)
- medium_58_modified_for_dsm_24661 (CultureMech:006047)
- medium_58_modified_for_dsm_7198 (CultureMech:006048)
- medium_58_modified_for_dsm_7199 (CultureMech:006049)
- medium_58_modified_for_dsm_7200 (CultureMech:006050)
- medium_58_modified_for_dsm_7201 (CultureMech:006051)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:10.062641Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 58 |
| 2026-01-28T19:48:37.937813Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:35.296055Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:06.653299Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001717 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.864701+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BIFIDOBACTERIUM MEDIUM" to "bifidobacterium_medium" for consistent matching |
| 2026-06-05T07:40:50.998967+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.907761+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:25.421451+00:00 | recipe-merger | Merged 28 duplicate recipes into canonical record | Sources: bifidobacterium_medium, medium_58_modified_for_dsm_16839, medium_58_modified_for_dsm_16840, medium_58_modified_for_dsm_16980, medium_58_modified_for_dsm_16981, medium_58_modified_for_dsm_17042, medium_58_modified_for_dsm_17774, medium_58_modified_for_dsm_17775, medium_58_modified_for_dsm_17776, medium_58_modified_for_dsm_17777, medium_58_modified_for_dsm_17778, medium_58_modified_for_dsm_17779, medium_58_modified_for_dsm_20461, medium_58_modified_for_dsm_20462, medium_58_modified_for_dsm_20465, medium_58_modified_for_dsm_20466, medium_58_modified_for_dsm_20467, medium_58_modified_for_dsm_20756, medium_58_modified_for_dsm_20757, medium_58_modified_for_dsm_20762, medium_58_modified_for_dsm_20764, medium_58_modified_for_dsm_20765, medium_58_modified_for_dsm_21655, medium_58_modified_for_dsm_24661, medium_58_modified_for_dsm_7198, medium_58_modified_for_dsm_7199, medium_58_modified_for_dsm_7200, medium_58_modified_for_dsm_7201 |
| 2026-08-06T03:05:25.421636+00:00 | merge_recipes.py | MERGED_RECIPES | merged 28 source recipe(s) on fingerprint 7368a3f1cd6e544362f37add9e3a10c2f296466dbc5597863cc79cdb15a1d5a9 |