halomonas_subglaciescola

CultureMech:001730 bacterial COMPLEX LIQUID
Original name: HALOMONAS SUBGLACIESCOLA

Overview

External ontology
mediadive.medium:602 (source link unavailable) — HALOMONAS SUBGLACIESCOLA
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'halomonas_subglaciescola_artificial_organic_lake_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:602', 'original_category': 'bacterial'}
pH
8.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium602.pdf

Composition

flowchart LR
    CultureMech_001730["halomonas_subglaciescola"]
    ing0_NaCl["NaCl"]
    CultureMech_001730 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001730 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_KCl["KCl"]
    CultureMech_001730 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001730 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001730 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_KNO3["KNO3"]
    CultureMech_001730 --> ing5_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing5_KNO3 -.-> CHEBI_63043
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001730 --> ing6_Yeast_extract
    ing7_Vitamin_B12["Vitamin B12"]
    CultureMech_001730 --> ing7_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing7_Vitamin_B12 -.-> CHEBI_176843
    ing8_Biotin["Biotin"]
    CultureMech_001730 --> ing8_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing8_Biotin -.-> CHEBI_15956
    ing9_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001730 --> ing9_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing9_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing10_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001730 --> ing10_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing10_Calcium_pantothenate -.-> CHEBI_31345
    ing11_Folic_acid["Folic acid"]
    CultureMech_001730 --> ing11_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing11_Folic_acid -.-> CHEBI_27470
    ing12_Nicotinamide["Nicotinamide"]
    CultureMech_001730 --> ing12_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing12_Nicotinamide -.-> CHEBI_17154
    ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001730 --> ing13_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing14_K2HPO4["K2HPO4"]
    CultureMech_001730 --> ing14_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing14_K2HPO4 -.-> CHEBI_131527
    ing15_KH2PO4["KH2PO4"]
    CultureMech_001730 --> ing15_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing15_KH2PO4 -.-> CHEBI_63036
    ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001730 --> ing16_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing17__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_001730 --> ing17__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing17__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001730 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_Na_EDTA["Na-EDTA"]
    CultureMech_001730 --> ing19_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing19_Na_EDTA -.-> CHEBI_64734
    ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001730 --> ing20_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing21_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001730 --> ing21_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing21_MnSO4_x_H2O -.-> CHEBI_86364
    ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001730 --> ing22_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing23_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001730 --> ing23_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing23_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing24_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_001730 --> ing24_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing24_Na2B4O7_x_10_H2O -.-> CHEBI_131366

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
80 G_PER_L CHEBI:26710
sodium chloride
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
39.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 9.5, 29.7]
3 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.535 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.2, 3.335]
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.1 G_PER_L CHEBI:62946
ammonium sulfate
6 KNO3
K.NO3 101.102 g/mol
0.1 G_PER_L CHEBI:63043
potassium nitrate
7 Yeast extract 1 G_PER_L
8 Vitamin B12 0.01 G_PER_L CHEBI:176843
vitamin B12
9 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
10 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
11 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
12 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
13 Nicotinamide
C6H6N2O 122.127 g/mol
0.005 G_PER_L CHEBI:17154
nicotinamide
14 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
15 K2HPO4
HO4P.2K 174.174 g/mol
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
16 KH2PO4
H2O4P.K 136.084 g/mol
2.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
17 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
18 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
19 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.099, 0.5]
20 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
21 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
22 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.154 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
23 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
24 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
25 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate

Preparation

  1. pH 8.0
  2. Autoclave and add, on cooling 20.0 ml sterile Hutner's salts (see medium 590), 1.0 ml vitamin solution, and 20.0 ml sterile phosphate supplement.
  3. Filter-sterilized.
  4. Dissolve the nitrilotriacetic acid.
  5. Adjust pH to 7.0 with KOH (~7.3 g).
  6. Dissolve other salts separately and combine.
  7. Adjust pH to 6.8 with NaOH or H2SO4.
  8. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:11.700221Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 602
2026-03-13T03:08:45.460099Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:06.782582Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001730
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.005037+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOMONAS SUBGLACIESCOLA" to "halomonas_subglaciescola" for consistent matching
2026-06-05T07:41:07.904041+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.953223+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:59.561234+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:37.259547+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: halomonas_subglaciescola, halomonas_subglaciescola_artificial_organic_lake_medium
2026-08-06T03:05:37.259586+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint cb668ec421b94589b427b2567a018e4f18325d16070265939156351f36547a03