Overview
- External ontology
- mediadive.medium:616 (source link unavailable) — ALKALITHERMOBACTER MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_616_modified_for_dsm_8682', 'source': 'KOMODO', 'source_id': 'komodo.medium:616_8682', 'original_category': 'bacterial'}
- pH
- 10.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium616.pdf
Composition
flowchart LR
CultureMech_001745["alkalithermobacter_medium"]
ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_001745 --> ing0_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing1_KCl["KCl"]
CultureMech_001745 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_Yeast_extract["Yeast extract"]
CultureMech_001745 --> ing2_Yeast_extract
ing3_Tryptone["Tryptone"]
CultureMech_001745 --> ing3_Tryptone
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_001745 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_Na2CO3["Na2CO3"]
CultureMech_001745 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_D_Glucose["D-Glucose"]
CultureMech_001745 --> ing6_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing6_D_Glucose -.-> CHEBI_17634
ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001745 --> ing7_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001745 --> ing8_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.36 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.08 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 | Yeast extract | 5 G_PER_L | ||
| 4 | Tryptone | 10 G_PER_L | ||
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
5.3 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
D-Glucose
C6H12O6 180.156 g/mol
|
3 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 8 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.2 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 9 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.2 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Dissolve ingredients (except carbonate, glucose, cysteine and sulfide) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and adjust pH to 10 by adding sterile anoxic 3 N NaOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- alkalithermobacter_medium (CultureMech:001745)
- medium_616_modified_for_dsm_8682 (CultureMech:006107)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.745703Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 616 |
| 2026-03-13T06:19:06.922026Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001745 |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.156870+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALITHERMOBACTER MEDIUM" to "alkalithermobacter_medium" for consistent matching |
| 2026-06-05T07:40:48.223884+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.923402+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:22.692626+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: alkalithermobacter_medium, medium_616_modified_for_dsm_8682 |
| 2026-08-06T03:05:22.692664+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 4de45f4ca4f96ec85cb5c2c2a444bab65228343605d57b62388d06e24af9f223 |