Overview
- External ontology
- mediadive.medium:620 (source link unavailable) — 2% MH MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium620.pdf
Composition
flowchart LR
CultureMech_001751["2_mh_medium"]
ing0_Glucose["Glucose"]
CultureMech_001751 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_KH2PO4["KH2PO4"]
CultureMech_001751 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_001751 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001751 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_NaCl["NaCl"]
CultureMech_001751 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001751 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_Methanol["Methanol"]
CultureMech_001751 --> ing6_Methanol
CHEBI_17790["CHEBI:17790"]
ing6_Methanol -.-> CHEBI_17790
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
1.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.025 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
0.5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.002 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
Methanol
CH4O 32.042 g/mol
|
20 G_PER_L |
CHEBI:17790
methanol
|
Preparation
- pH 7-7.5
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- 2_mh_medium (CultureMech:001751)
- KOMODO_620_2_MH_MEDIUM (CultureMech:006115)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.754287Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 620 |
| 2026-03-13T06:19:06.949204Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001751 |
| 2026-03-17T06:30:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.189976+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "2% MH MEDIUM" to "2_mh_medium" for consistent matching |
| 2026-05-20T06:00:46.876250+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-7.5' -> {'min': 7.0, 'max': 7.5} |
| 2026-06-05T07:40:15.758614+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.537571+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:34.040528+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: 2_mh_medium, KOMODO_620_2_MH_MEDIUM |
| 2026-08-06T03:04:34.040563+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint ebea5cbd0c1784916cd17463cc4342d6e3dd404bf6e6b9dc2710bc795af3fbd5 |