Overview
- External ontology
- mediadive.medium:628 (source link unavailable) — MMB MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium628.pdf
Composition
flowchart LR
CultureMech_001759["mmb_medium"]
ing0_Peptone["Peptone"]
CultureMech_001759 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001759 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_001759 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_001759 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Vitamin_B12["Vitamin B12"]
CultureMech_001759 --> ing4_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing4_Vitamin_B12 -.-> CHEBI_176843
ing5_Biotin["Biotin"]
CultureMech_001759 --> ing5_Biotin
CHEBI_15956["CHEBI:15956"]
ing5_Biotin -.-> CHEBI_15956
ing6_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_001759 --> ing6_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing6_Calcium_pantothenate -.-> CHEBI_31345
ing7_Folic_acid["Folic acid"]
CultureMech_001759 --> ing7_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing7_Folic_acid -.-> CHEBI_27470
ing8_Nicotinamide["Nicotinamide"]
CultureMech_001759 --> ing8_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing8_Nicotinamide -.-> CHEBI_17154
ing9_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001759 --> ing9_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing9_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing10_Riboflavin["Riboflavin"]
CultureMech_001759 --> ing10_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing10_Riboflavin -.-> CHEBI_17015
ing11_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001759 --> ing11_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing11_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001759 --> ing12_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001759 --> ing13_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001759 --> ing14_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
ing15__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_001759 --> ing15__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing15__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001759 --> ing16_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
ing17_Na_EDTA["Na-EDTA"]
CultureMech_001759 --> ing17_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing17_Na_EDTA -.-> CHEBI_64734
ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001759 --> ing18_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing19_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001759 --> ing19_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing19_MnSO4_x_H2O -.-> CHEBI_86364
ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001759 --> ing20_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
ing21_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_001759 --> ing21_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing21_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
ing22_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_001759 --> ing22_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing22_Na2B4O7_x_10_H2O -.-> CHEBI_131366
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 0.15 G_PER_L | ||
| 2 | Yeast extract | 0.15 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.25 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 6 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 7 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 8 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 9 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.005 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 10 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.005 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 11 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 12 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 13 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 14 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
29.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 15 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.335 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 16 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
0.00925 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 17 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.599 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.099, 0.5] |
| 18 |
Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.25 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 19 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.095 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 20 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.154 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 21 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.0392 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 22 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.0248 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
|
| 23 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0177 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Preparation
- pH 7.2
- Glucose and vitamin solution should be filter-sterilized separately.
- Dissolve the nitrilotriacetic acid.
- Adjust pH to 7.0 with KOH (~7.3 g).
- Dissolve other salts separately and combine.
- Adjust pH to 6.8 with NaOH or H2SO4.
- Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- DSMZ_628_MMB_MEDIUM (CultureMech:001759)
- KOMODO_628_MMB_medium (CultureMech:006134)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.773020Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 628 |
| 2026-03-13T03:09:10.469906Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:07.007013Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001759 |
| 2026-03-17T06:30:17.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.257850+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMB MEDIUM" to "mmb_medium" for consistent matching |
| 2026-06-05T07:40:16.212324+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.646671+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:34.776518+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: DSMZ_628_MMB_MEDIUM, KOMODO_628_MMB_medium |
| 2026-08-06T03:04:34.776551+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint ba7144d5fd6b2fc38b60efabdd199c232b13a3950713aceeea6016f80e158de7 |