Overview
- External ontology
- mediadive.medium:634b (source link unavailable) — RCM MEDIUM (with Sea Salts)
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium634b.pdf
Composition
flowchart LR
CultureMech_001767["rcm_medium_with_sea_salts"]
ing0_dehydrated_RCM_medium["dehydrated RCM medium"]
CultureMech_001767 --> ing0_dehydrated_RCM_medium
ing1_Sea_salts["Sea salts"]
CultureMech_001767 --> ing1_Sea_salts
ing2_Sodium_resazurin["Sodium resazurin"]
CultureMech_001767 --> ing2_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Sodium_resazurin -.-> CHEBI_8806
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | dehydrated RCM medium | 38 G_PER_L | ||
| 2 | Sea salts | 30 G_PER_L | ||
| 3 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
Preparation
- Dissolve dehydrated RCM medium (available from BD BBL, OXOID, or MERCK) and SIGMA Sea Salts (30.0 g/l) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v). Bring to a boil and cool to room temperature while sparging with 100% N2 gas. Dispense the medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave for 15 min at 121°C. Adjust the pH of the complete medium to 6.8.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- rcm_medium_with_sea_salts (CultureMech:001767)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.790420Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 634b |
| 2026-03-13T06:19:07.055536Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001767 |
| 2026-03-17T19:45:19.314489+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RCM MEDIUM (with Sea Salts)" to "rcm_medium_with_sea_salts" for consistent matching |
| 2026-08-06T03:05:54.374160+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5cb1b5ffabae0afc0896e254f87b819d950df385c8dc194c0eb6e2ddda9a2a03 |