Overview
- External ontology
- mediadive.medium:635 (source link unavailable) — GRACILINEMA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium635.pdf
Composition
flowchart LR
CultureMech_001769["gracilinema_medium"]
ing0_D_Glucose["D-Glucose"]
CultureMech_001769 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Tryptone["Tryptone"]
CultureMech_001769 --> ing1_Tryptone
ing2_Sodium_resazurin["Sodium resazurin"]
CultureMech_001769 --> ing2_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Sodium_resazurin -.-> CHEBI_8806
ing3_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001769 --> ing3_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing3_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing4_HCl["HCl"]
CultureMech_001769 --> ing4_HCl
CHEBI_17883["CHEBI:17883"]
ing4_HCl -.-> CHEBI_17883
ing5_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001769 --> ing5_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing5_FeCl2_x_4_H2O -.-> CHEBI_86249
ing6_ZnCl2["ZnCl2"]
CultureMech_001769 --> ing6_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing6_ZnCl2 -.-> CHEBI_49976
ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001769 --> ing7_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
ing8_H3BO3["H3BO3"]
CultureMech_001769 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001769 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001769 --> ing10_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing10_CuCl2_x_2_H2O -.-> CHEBI_86318
ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001769 --> ing11_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001769 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001769 --> ing13_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001769 --> ing14_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
ing15_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001769 --> ing15_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing15_MnSO4_x_H2O -.-> CHEBI_86364
ing16_NaCl["NaCl"]
CultureMech_001769 --> ing16_NaCl
CHEBI_26710["CHEBI:26710"]
ing16_NaCl -.-> CHEBI_26710
ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001769 --> ing17_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
ing18_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001769 --> ing18_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing18_CoSO4_x_7_H2O -.-> CHEBI_91244
ing19_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001769 --> ing19_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing19_CaCl2_x_2_H2O -.-> CHEBI_86158
ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001769 --> ing20_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001769 --> ing21_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
ing22_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001769 --> ing22_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing22_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001769 --> ing23_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001769 --> ing24_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing25_Biotin["Biotin"]
CultureMech_001769 --> ing25_Biotin
CHEBI_15956["CHEBI:15956"]
ing25_Biotin -.-> CHEBI_15956
ing26_Folic_acid["Folic acid"]
CultureMech_001769 --> ing26_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing26_Folic_acid -.-> CHEBI_27470
ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001769 --> ing27_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing28_Thiamine_HCl["Thiamine HCl"]
CultureMech_001769 --> ing28_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing28_Thiamine_HCl -.-> CHEBI_49105
ing29_Riboflavin["Riboflavin"]
CultureMech_001769 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_001769 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
1.98807 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 2 | Tryptone | 1.98807 G_PER_L | ||
| 3 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000497018 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 4 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.248509 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 5 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 6 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 7 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 8 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.016 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.006, 0.01] |
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 11 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 12 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.054 G_PER_L |
CHEBI:34887
nickel dichloride
|
[Merged 2 duplicates: 0.024, 0.03] |
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.046 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
[Merged 2 duplicates: 0.036, 0.01] |
| 14 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 15 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 16 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 17 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 18 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 19 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 20 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 21 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 22 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 23 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 24 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 25 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 26 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 27 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 28 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 29 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 31 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 32 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 33 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 34 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 35 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except cysteine and vitamins), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Then add cysteine, adjust pH to 7.0, dispense medium under the same gas atmosphere in anoxic Hungate-type tubes or serum vials, and autoclave. After sterilization, add vitamins from a sterile anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Check the pH of the complete medium and adjust to 7.0 if necessary.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- gracilinema_medium (CultureMech:001769)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.792688Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 635 |
| 2026-02-06T05:10:38.614630Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:19.163561Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:07.070614Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001769 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.330025+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GRACILINEMA MEDIUM" to "gracilinema_medium" for consistent matching |
| 2026-06-05T07:41:06.677028+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.167422+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:36.462080+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 840b4ddbb15d8b1b11f73b03ccbbe7e0d904986a7371cb8f7cb4c7c3ef1b5a7f |