Overview
- External ontology
- mediadive.medium:664 (source link unavailable) — PSEUDOTHERMOTOGA ELFII MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium664.pdf
Composition
flowchart LR
CultureMech_001802["pseudothermotoga_elfii_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_001802 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_K2HPO4["K2HPO4"]
CultureMech_001802 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_KH2PO4["KH2PO4"]
CultureMech_001802 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001802 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001802 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_KCl["KCl"]
CultureMech_001802 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_NaCl["NaCl"]
CultureMech_001802 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Na_acetate["Na-acetate"]
CultureMech_001802 --> ing7_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing7_Na_acetate -.-> CHEBI_32954
ing8_Yeast_extract["Yeast extract"]
CultureMech_001802 --> ing8_Yeast_extract
ing9_Trypticase_peptone["Trypticase peptone"]
CultureMech_001802 --> ing9_Trypticase_peptone
ing10_Sodium_resazurin["Sodium resazurin"]
CultureMech_001802 --> ing10_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Sodium_resazurin -.-> CHEBI_8806
ing11_Na2CO3["Na2CO3"]
CultureMech_001802 --> ing11_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing11_Na2CO3 -.-> CHEBI_29377
ing12_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_001802 --> ing12_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing12_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing13_D_Glucose["D-Glucose"]
CultureMech_001802 --> ing13_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing13_D_Glucose -.-> CHEBI_17634
ing14_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001802 --> ing14_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing14_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing15_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001802 --> ing15_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing15_Na2S_x_9_H2O -.-> CHEBI_76209
ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001802 --> ing16_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
ing17_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001802 --> ing17_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing17_MgSO4_x_7_H2O -.-> CHEBI_31795
ing18_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001802 --> ing18_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing18_MnSO4_x_H2O -.-> CHEBI_86364
ing19_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001802 --> ing19_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing19_FeSO4_x_7_H2O -.-> CHEBI_75836
ing20_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001802 --> ing20_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing20_CoSO4_x_7_H2O -.-> CHEBI_91244
ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001802 --> ing21_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001802 --> ing22_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
ing23_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001802 --> ing23_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing23_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing24_H3BO3["H3BO3"]
CultureMech_001802 --> ing24_H3BO3
CHEBI_33118["CHEBI:33118"]
ing24_H3BO3 -.-> CHEBI_33118
ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001802 --> ing25_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing26_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001802 --> ing26_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing26_NiCl2_x_6_H2O -.-> CHEBI_34887
ing27_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001802 --> ing27_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing27_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing28_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001802 --> ing28_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing28_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.990099 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.29703 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.29703 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.19802 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.19900990000000002 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.0990099, 0.1] |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.0990099 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
10.90099 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 9.90099, 1.0] |
| 8 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.49505 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 9 | Yeast extract | 4.9505 G_PER_L | ||
| 10 | Trypticase peptone | 4.9505 G_PER_L | ||
| 11 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.00049505 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.48515 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 13 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
4.9505 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 14 |
D-Glucose
C6H12O6 180.156 g/mol
|
3.9604 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 15 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.49505 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 16 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.49505 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 17 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 18 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 19 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 20 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 21 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 22 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 23 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 24 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 25 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 26 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 27 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 28 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 29 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve ingredients except carbonate, thiosulfate, glucose and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add thiosulfate, glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The pH of the complete medium should be adjusted to 7.5, if necessary.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pseudothermotoga_elfii_medium (CultureMech:001802)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.867340Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 664 |
| 2026-03-13T03:08:44.144317Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:07.314877Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001802 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.605331+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PSEUDOTHERMOTOGA ELFII MEDIUM" to "pseudothermotoga_elfii_medium" for consistent matching |
| 2026-06-05T07:41:42.761017+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.484189+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.667009+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 119056f93746a23b1020c22f16f4e09583c018f3cd4823b317e81f829db35c4e |