Overview
- External ontology
- mediadive.medium:666b (source link unavailable) — METHANOMICROBIUM MOBILE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: DSMZ
Composition
flowchart LR
CultureMech_001807["methanomicrobium_mobile_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_001807 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_KH2PO4["KH2PO4"]
CultureMech_001807 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NaCl["NaCl"]
CultureMech_001807 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_001807 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001807 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001807 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_Indigocarmine["Indigocarmine"]
CultureMech_001807 --> ing6_Indigocarmine
CHEBI_31695["CHEBI:31695"]
ing6_Indigocarmine -.-> CHEBI_31695
ing7_NaHCO3["NaHCO3"]
CultureMech_001807 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Yeast_extract["Yeast extract"]
CultureMech_001807 --> ing8_Yeast_extract
ing9_DL_Dithiothreitol["DL-Dithiothreitol"]
CultureMech_001807 --> ing9_DL_Dithiothreitol
CHEBI_18320["CHEBI:18320"]
ing9_DL_Dithiothreitol -.-> CHEBI_18320
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001807 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.23 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.23 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.45 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.45 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.06 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.09 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
Indigocarmine
C16H8N2O8S2.2Na 466.36 g/mol
|
0.005 G_PER_L |
CHEBI:31695
indigo carmine
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
6.4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 | Yeast extract | 5 G_PER_L | ||
| 10 |
DL-Dithiothreitol
C4H10O2S2 154.256 g/mol
|
0.3 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Dissolve ingredients (except bicarbonate, cysteine, and DTT), bring the medium to a boil, and then cool it to room temperature under an 80% H2 and 20% CO2 gas atmosphere. Add the bicarbonate and equilibrate the medium to pH 6.8. Distribute under the same gas atmosphere into anoxic Hungate-type tubes or serum vials, filling them to 30% of their volume, and then autoclave. After that, add cysteine and DTT from sterile anoxic stock solutions prepared under a 100% N2 gas atmosphere. Adjust the pH of the complete medium to 6.7 - 6.8, if necessary.
- After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar of overpressure.
- Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methanomicrobium_mobile_medium (CultureMech:001807)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.882377Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 666b |
| 2026-03-13T06:19:07.356480Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001807 |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.655858+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOMICROBIUM MOBILE MEDIUM" to "methanomicrobium_mobile_medium" for consistent matching |
| 2026-06-05T07:41:32.027050+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.162230+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:30.864840+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0da37a4aa1e823447656bce3bac739d8ade7c3b86b8abc390f56aa5d32556855 |