Overview
- External ontology
- mediadive.medium:68 (source link unavailable) — HALOTHIOBACILLUS NEAPOLITANUS MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'thiobacillus_neapolitanus_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:68', 'original_category': 'bacterial'}
- pH range
- {'min': 6.6, 'max': 7.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium68.pdf
Composition
flowchart LR
CultureMech_001826["halothiobacillus_neapolitanus_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001826 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_001826 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001826 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_NH4Cl["NH4Cl"]
CultureMech_001826 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_Bromocresol_purple["Bromocresol purple"]
CultureMech_001826 --> ing4_Bromocresol_purple
CHEBI_86154["CHEBI:86154"]
ing4_Bromocresol_purple -.-> CHEBI_86154
ing5_Agar["Agar"]
CultureMech_001826 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_001826 --> ing6_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing6_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing7_Na2_EDTA["Na2-EDTA"]
CultureMech_001826 --> ing7_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing7_Na2_EDTA -.-> CHEBI_64734
ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001826 --> ing8_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001826 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001826 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001826 --> ing11_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
ing12__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_001826 --> ing12__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing12__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001826 --> ing13_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001826 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
4 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.8 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.4 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
Bromocresol purple
C21H16Br2O5S 540.229 g/mol
|
0.5 G_PER_L |
CHEBI:86154
bromocresol purple
|
|
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Optional ingredient (for solid medium) |
| 7 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
10 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 8 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
50 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 9 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
5.54 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
5.06 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 13 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
1.1 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 14 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
1.57 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.61 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Preparation
- Dissolve ingredients except thiosulfate, dispense into suitable cultivation vessels and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution. Adjust pH of complete medium to 6.6 - 7.0.
- Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- halothiobacillus_neapolitanus_medium (CultureMech:001826)
- thiobacillus_neapolitanus_medium (CultureMech:006294)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.081867Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 68 |
| 2026-03-13T06:19:07.527478Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001826 |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.850079+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOTHIOBACILLUS NEAPOLITANUS MEDIUM" to "halothiobacillus_neapolitanus_medium" for consistent matching |
| 2026-05-20T06:01:22.410264+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.6-7' -> {'min': 6.6, 'max': 7.0} |
| 2026-06-05T07:41:08.276624+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.692617+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:37.400009+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: halothiobacillus_neapolitanus_medium, thiobacillus_neapolitanus_medium |
| 2026-08-06T03:05:37.400054+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 05b0fdee813d50b9ba77bf07fc7277fd286c03184a6eb97d040a22a95be36632 |