acidithiobacillus_ferrooxidans_medium

CultureMech:001847 bacterial DEFINED LIQUID
Original name: ACIDITHIOBACILLUS FERROOXIDANS MEDIUM

Overview

External ontology
mediadive.medium:70 (source link unavailable) — ACIDITHIOBACILLUS FERROOXIDANS MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'thiobacillus_ferrooxidans_medium_with_ferrous_sulfate', 'source': 'KOMODO', 'source_id': 'komodo.medium:70', 'original_category': 'bacterial'}
pH
1.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium70.pdf

Composition

flowchart LR
    CultureMech_001847["acidithiobacillus_ferrooxidans_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001847 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001847 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001847 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001847 --> ing3_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing4_H2SO4["H2SO4"]
    CultureMech_001847 --> ing4_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing4_H2SO4 -.-> CHEBI_26836

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.4 G_PER_L CHEBI:62946
ammonium sulfate
4 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
33.3 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
5 H2SO4
H2O4S 98.079 g/mol
1000 G_PER_L CHEBI:26836
sulfuric acid

Preparation

  1. Adjust pH to 1.8 with 2N sulfuric acid. Sterilize by autoclaving for 15 min at 121°C.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:10.086927Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 70
2026-03-13T06:19:07.703002Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001847
2026-03-17T06:30:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.045827+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ACIDITHIOBACILLUS FERROOXIDANS MEDIUM" to "acidithiobacillus_ferrooxidans_medium" for consistent matching
2026-06-05T07:40:47.353381+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.617238+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:21.991377+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: acidithiobacillus_ferrooxidans_medium, thiobacillus_ferrooxidans_medium_with_ferrous_sulfate
2026-08-06T03:05:21.991424+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint fd9133adba69616a0e7f496435f9d7191a5ff792b7fb4cbab2c74947d2bc5968